Šćepanović Maja, Košćak Laura, Šoštarčić Valentina, Pismarović Laura, Milanović-Litre Ana, Kljak Kristina
University of Zagreb Faculty of Agriculture, Svetošimunska 25, 10000 Zagreb, Croatia.
Institute for Agriculture and Tourism, Karla Huguesa 8, 52440 Poreč, Croatia.
Biology (Basel). 2022 Mar 22;11(4):482. doi: 10.3390/biology11040482.
This study aimed to investigate whether different doses of specific phenolic acids (chlorogenic acid, caffeic acid, ferulic acid, gallic acid, protocatechuic acid, -hydroxybenzoic acid, syringic acid, vanillic acid, and -coumaric acid), alone or in combination, can inhibit the early growth of the common ragweed ( L., Asterales: Asteraceae). A seed bioassay was performed in Petri dishes and placed in a climate chamber to assess the effects of five dose levels of phenolic acids to radicle and shoot length, as well seedling biomass of . The lowest dose of phenolic acid corresponded to the natural phenolic acid concentration previously reported in dry plant tissue samples from Brassicaceae cover crop plants. Results show that the inhibition of the early growth of depends strongly on phenolic acid. Across different treatments, high doses of phenolic acids significantly shortened shoots and radicles, as well as reduced seedling biomass. Treating seeds with ferulic acid alone, vanillic acid alone, -hydroxybenzoic acid alone, or a mixture of all phenolic acids significantly reduced all early growth parameters. The estimated effective dose for the 50% inhibition (ED) of radicle growth in seedlings was 368.39 ± 59.85 × 10 mol with ferulic acid, 135.41 ± 17.65 × 10 mol with -coumaric acid, 810.36 ± 134.15 × 10 mol with -hydroxybenzoic acid, and 160.11 ± 12.30 × 10 mol with the combination of all phenolic acids.
本研究旨在调查不同剂量的特定酚酸（绿原酸、咖啡酸、阿魏酸、没食子酸、原儿茶酸、对羟基苯甲酸、丁香酸、香草酸和对香豆酸）单独或组合使用时，是否能够抑制豚草（Ambrosia artemisiifolia L.，菊目：菊科）的早期生长。在培养皿中进行种子生物测定，并将其置于气候箱中，以评估酚酸的五个剂量水平对豚草胚根和芽长度以及幼苗生物量的影响。酚酸的最低剂量对应于先前在十字花科覆盖作物植物的干燥植物组织样本中报道的天然酚酸浓度。结果表明，豚草早期生长的抑制强烈依赖于酚酸。在不同处理中，高剂量的酚酸显著缩短了芽和胚根，同时降低了幼苗生物量。单独用阿魏酸、香草酸、对羟基苯甲酸处理种子，或使用所有酚酸的混合物，均显著降低了所有早期生长参数。在豚草幼苗中，胚根生长50%抑制（ED50）的估计有效剂量，阿魏酸为368.39±59.85×10⁻⁶mol，对香豆酸为135.41±17.65×10⁻⁶mol，对羟基苯甲酸为810.36±134.15×10⁻⁶mol，所有酚酸组合为160.11±12.30×10⁻⁶mol。
