Jia Xiaojiang, Xiong Xinghua, Chen Hao, Xiao Gang, Cheng Qian, Zhang Zhenqian
College of Agriculture, Hunan Agricultural University, Changsha 410128, China.
Junlebao Dairy Co., Ltd., Shijiazhuang 050221, China.
Biology (Basel). 2022 Mar 22;11(4):483. doi: 10.3390/biology11040483.
In this study, lysine acetylation analysis was conducted using two near-isogenic lines, HOCR and LOCR, containing high and low oleic acid contents, respectively, to explore this relationship. Proteins showing differences in quantitative information between the lines were identified in lysine acetylation analysis, and KEGG pathways were analyzed, yielding 45 enriched proteins, most of which are involved in carbon fixation in photosynthetic organisms, photosynthesis, ascorbate and aldarate metabolism, and glycolysis. Potential key genes related to fatty acid metabolisms were determined. To further explore the effect of acetylation modification on fatty acid metabolisms, the acyl-ACP3 related gene was cloned, and a base mutation at No.63 was changed via overlapping primer PCR method. This study is the first to demonstrate that acetylation modification can regulate oleic acid metabolisms, which provides a promising approach for the study of the molecular mechanism of oleic acid in rapeseed.
在本研究中，使用两个分别含有高油酸和低油酸含量的近等基因系HOCR和LOCR进行赖氨酸乙酰化分析，以探究这种关系。在赖氨酸乙酰化分析中鉴定出两系之间定量信息存在差异的蛋白质，并对KEGG途径进行了分析，产生了45种富集蛋白，其中大多数参与光合生物中的碳固定、光合作用、抗坏血酸和醛糖代谢以及糖酵解。确定了与脂肪酸代谢相关的潜在关键基因。为了进一步探究乙酰化修饰对脂肪酸代谢的影响，克隆了酰基-ACP3相关基因，并通过重叠引物PCR方法改变了第63位的碱基突变。本研究首次证明乙酰化修饰可调节油酸代谢，为油菜籽中油酸分子机制的研究提供了一种有前景的方法。
