Pang Yaru, Wu Ruobin, Cui Tianlin, Zhang Zequn, Dong Li, Chen Fang, Hu Xiaosong
College of Food Science and Nutritional Engineering, China Agricultural University, Beijing 100083, China.
China National Engineering Research Center for Fruit and Vegetable Processing, Beijing 100083, China.
Foods. 2022 Apr 13;11(8):1123. doi: 10.3390/foods11081123.
In this study, a method of spore inactivation under high pressure (P, 200 MPa) combined with moderate temperature (T, 80 °C) and the addition of antimicrobial peptide LK (10 μg/mL) was investigated. Spores presented cortex hydrolysis and inner membrane (IM) damage with an 8.16 log reduction in response to treatment with PT-LK, as observed by phase-contrast and inverted fluorescence microscopy and flow cytometry (FCM) analysis. Furthermore, a tandem mass tag (TMT) quantitative proteomics approach was utilized because Liquid chromatography-tandem mass spectrometry (LC-MS/MS) analysis data were used. After treatment with PT-LK, 17,017 polypeptides and 3166 proteins were detected from spores. Among them, 78 proteins showed significant differences in abundance between the PT-LK-treated and control groups, with 49 proteins being upregulated and 29 proteins being downregulated in the PT-LK-treated group. Genetic information processing, metabolism, cellular process, and environmental information processing were the main mechanisms of PT-LK-mediated spore inactivation.
在本研究中，研究了一种在高压（P，200MPa）、适度温度（T，80°C）并添加抗菌肽LK（10μg/mL）的条件下使孢子失活的方法。通过相差显微镜、倒置荧光显微镜和流式细胞术（FCM）分析观察到，经PT-LK处理后，孢子出现皮层水解和内膜（IM）损伤，孢子数量减少了8.16个对数级。此外，由于使用了液相色谱-串联质谱（LC-MS/MS）分析数据，因此采用了串联质量标签（TMT）定量蛋白质组学方法。经PT-LK处理后，从孢子中检测到17,017种多肽和3166种蛋白质。其中，78种蛋白质在PT-LK处理组和对照组之间的丰度存在显著差异，PT-LK处理组中有49种蛋白质上调，29种蛋白质下调。遗传信息处理、代谢、细胞过程和环境信息处理是PT-LK介导的孢子失活的主要机制。
