Mu Kangyi, Cui Tianlin, Zhang Zequn, Shi Yicong, Fang Chen, Dong Li, Hu Xiaosong
Key Laboratory of Fruits and Vegetables Processing, Ministry of Agriculture, Engineering Research Centre for Fruits and Vegetables Processing, Ministry of Education, National, Engineering Research Center for Fruit and Vegetable Processing, College of Food Science and Nutritional Engineering, China Agricultural University, Beijing 100083, China.
College of Food Science and Technology, Henan Agricultural University, Zhengzhou 450002, China.
Microorganisms. 2025 Mar 26;13(4):744. doi: 10.3390/microorganisms13040744.
In the food industry, food spoilage caused by spores is a pressing scientific challenge that needs to be addressed urgently, and spore germination is a key approach to solving this problem. Studies have shown that peptidoglycan-induced spore germination represents a novel mechanism of action, which can bind to the PASTA domain of the serine/threonine kinase PrkC. However, the signaling mechanism of peptidoglycan-induced spore germination remains unclear. This study focuses on , using pull-down experiments to screen for proteins interacting with PrkC. There are 80 interaction proteins of PrkC that were identified in the spore. GO analysis reveals that PrkC-interacting proteins in the spore are mainly involved in metabolic processes, cell part and catalysis. KEGG results indicate that PrkC-interacting proteins in the spore are mainly involved in RNA degradation, quorum sensing, oxidative phosphorylation, etc. Additionally, proteins are categorized into six groups by function based on events that may be associated with post-germination triggered by peptidoglycan-induced activation of the PrkC signaling pathway, including "stimulate translation initiation" and "ATP synthesis and energy metabolism". The experimental results provide a theoretical basis for further elucidating the signaling mechanism of PrkC, revealing the signaling pathway of peptidoglycan-induced spore germination, and identifying targeted inducers and repressors of spore germination.
在食品工业中，由孢子引起的食品腐败是一个亟待解决的紧迫科学挑战，而孢子萌发是解决这一问题的关键途径。研究表明，肽聚糖诱导的孢子萌发代表了一种新的作用机制，它可以与丝氨酸/苏氨酸激酶PrkC的PASTA结构域结合。然而，肽聚糖诱导孢子萌发的信号传导机制仍不清楚。本研究聚焦于此，利用下拉实验筛选与PrkC相互作用的蛋白质。在孢子中鉴定出80种PrkC的相互作用蛋白。基因本体（GO）分析显示，孢子中与PrkC相互作用的蛋白主要参与代谢过程、细胞部分和催化作用。京都基因与基因组百科全书（KEGG）结果表明，孢子中与PrkC相互作用的蛋白主要参与RNA降解、群体感应、氧化磷酸化等过程。此外，根据可能与肽聚糖诱导的PrkC信号通路激活引发的萌发后事件相关的功能，将蛋白质分为六组，包括“刺激翻译起始”和“ATP合成与能量代谢”。实验结果为进一步阐明PrkC的信号传导机制、揭示肽聚糖诱导孢子萌发的信号通路以及鉴定孢子萌发的靶向诱导剂和抑制剂提供了理论依据。
