Gil Tae-Young, Kang Sung-Chul, Jin Bo-Ram, An Hyo-Jin
Department of Pharmacology, College of Korean Medicine, Sangji University, Wonju-si 26339, Gangwon-do, Korea.
Life (Basel). 2022 Apr 15;12(4):589. doi: 10.3390/life12040589.
Skin inflammation may cause allergic diseases such as allergic rhinitis, asthma, and atopic dermatitis. () is a member of the Euphorbiaceae family and is well-known for its anti-asthma effects. has traditionally been used to treat respiratory ailments, dysentery, jaundice, and digestive problems. However, its effects on skin inflammation remain unclear. Here, we determined the effects of 70% ethanol extract of leaves (ELE) in vitro using human keratinocyte HaCaT cells, which constitute most epidermal skin cells. We determined the inhibitory effects of ELE on the inflammation caused by tumor necrosis factor (TNF)-α/interferon (IFN)-γ in keratinocytes using ELISA, immunoblotting, and qRT-PCR assay. ELE was found to reduce the production and mRNA expression of pro-inflammatory cytokines such as TNF-α or interleukin-6 and the expression of various proteins, including signal transducers, activators of transcription 1/3, and mitogen-activated protein kinase. Expression levels of these proteins were found to be upregulated in the TNF-α/IFN-γ-stimulated condition and downregulated by ELE treatment. These results indicate that ELE protects HaCaT cells against TNF-α/IFN-γ-induced skin inflammation.
皮肤炎症可能会引发过敏性疾病，如过敏性鼻炎、哮喘和特应性皮炎。（）是大戟科的一员，以其抗哮喘作用而闻名。传统上一直用于治疗呼吸道疾病、痢疾、黄疸和消化问题。然而，其对皮肤炎症的影响仍不清楚。在此，我们使用构成大多数表皮皮肤细胞的人角质形成细胞HaCaT细胞，在体外确定了（）叶70%乙醇提取物（ELE）的作用。我们使用酶联免疫吸附测定（ELISA）、免疫印迹和定量逆转录聚合酶链反应（qRT-PCR）测定法，确定了ELE对角质形成细胞中由肿瘤坏死因子（TNF）-α/干扰素（IFN）-γ引起的炎症的抑制作用。发现ELE可减少促炎细胞因子如TNF-α或白细胞介素-6的产生和mRNA表达，以及包括信号转导子、转录激活因子1/3和丝裂原活化蛋白激酶在内的各种蛋白质的表达。发现这些蛋白质的表达水平在TNF-α/IFN-γ刺激条件下上调，而经ELE处理后下调。这些结果表明，ELE可保护HaCaT细胞免受TNF-α/IFN-γ诱导的皮肤炎症。
