用于尿微量样本中药物综合筛查的酶免疫测定法。
Enzyme immunoassay method for comprehensive drug screening in micro-samples of urine.
作者信息
Black D L, Goldberger B A, Caplan Y H
出版信息
Clin Chem. 1987 Mar;33(3):367-71.
We adapted the reagents from 11 different enzyme-multiplied immunoassay technique (EMIT; Syva Co., Palo Alto, CA 94304) drug-detection kits for use in a centrifugal analyzer. The antibody reagents were mixed into a single dilute solution, and the enzyme-labeled drug derivatives were combined similarly (Mixed EMIT reagents), for use in testing urine samples for the presence of multiple drugs. The assay, a rapid comprehensive drug-screening technique, requires 100 microL of sample and is capable of testing seven samples, in duplicate, simultaneously, in less than 10 min. Clinical evaluation (n = 325) by comparison with thin-layer chromatography (TLC) had the following results: 230 samples were negative by TLC and Mixed EMIT, 77 samples were positive by TLC and Mixed EMIT, 16 samples were negative by TLC and positive by Mixed EMIT, and two samples were positive by TLC and negative by Mixed EMIT.
我们对来自11种不同的酶放大免疫分析技术（EMIT；Syva公司，加利福尼亚州帕洛阿尔托94304）药物检测试剂盒的试剂进行了调整，以用于离心分析仪。将抗体试剂混合成单一的稀释溶液，酶标记的药物衍生物也以类似方式混合（混合EMIT试剂），用于检测尿液样本中多种药物的存在。该检测方法是一种快速的综合药物筛选技术，需要100微升样本，并且能够在不到10分钟的时间内同时对7个样本进行一式两份的检测。通过与薄层色谱法（TLC）比较进行的临床评估（n = 325）结果如下：230个样本经TLC和混合EMIT检测均为阴性，77个样本经TLC和混合EMIT检测均为阳性，16个样本经TLC检测为阴性但经混合EMIT检测为阳性，2个样本经TLC检测为阳性但经混合EMIT检测为阴性。
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