Hamlin C R
Institute of Pathology, Case Western Reserve University School of Medicine, Cleveland, OH 44106.
Clin Chem. 1988 Jan;34(1):158-62.
I have adapted, for use with a centrifugal analyzer, reagents from 10 different EMIT (Syva Co.) enzyme immunoassay urine drug-detection kits, reagents from another five serum kits, and reagents for determining ethanol. Assays for individual drugs in 100 microL of urine and in 100 microL of serum are performed simultaneously rather than sequentially in less than 15 min, with one-tenth the usual amount of reactants (sample + reagents). These assays, when combined with other easily performed tests, provide a rapid toxicology screen well-suited for evaluating acute emergency patients, and for performing a small number of daily routine screens. The total screen requires 500 microL of urine and 500 microL of serum. I summarize my experience with this procedure in its first year of use, and discuss differences between testing for the care of patients compared with searching for evidence of illegal drug use.
我对来自10种不同的酶倍增免疫测定法（Syva公司）尿液药物检测试剂盒、另外5种血清试剂盒的试剂以及用于测定乙醇的试剂进行了调整，以便与离心分析仪配合使用。在不到15分钟的时间内，同时而非依次对100微升尿液和100微升血清中的个别药物进行检测，反应剂（样品+试剂）用量仅为通常用量的十分之一。这些检测方法与其他易于操作的检测方法相结合，可提供一种快速毒理学筛查，非常适合评估急性急诊患者以及进行少量日常常规筛查。整个筛查需要500微升尿液和500微升血清。我总结了该方法使用第一年的经验，并讨论了在患者护理检测与查找非法药物使用证据检测之间的差异。
