Shih Yin-Hwa, Yu Cheng-Chia, Chang Kai-Chi, Tseng Yu-Hsin, Li Po-Jung, Hsia Shih-Min, Chiu Kuo-Chou, Shieh Tzong-Ming
Department of Healthcare Administration, Asia University, Taichung 41354, Taiwan.
School of Dentistry, Chung Shan Medical University, Taichung 40201, Taiwan.
Pharmaceuticals (Basel). 2022 Apr 18;15(4):488. doi: 10.3390/ph15040488.
5, 10, 15, 20-Tetrakis(3-hydroxyphenyl)chlorin (temoporfin) is a photosensitizer used in photodynamic therapy for oral cancer and periodontal disease treatment. This study determined the minimum inhibitory concentrations (MICs) and minimum bactericidal concentrations (MBCs) of temoporfin. Additionally, the combination of potassium iodide (KI) or antimicrobial agents in oral pathogens under hypoxic or normoxic conditions were determined. We also evaluated the biofilm removal effect and detected the expressions of the antibiotic resistance-related genes and biofilm formation-related genes of methicillin-resistant (MRSA). The results provided reveal that the combination of the temoporfin and KI had a synergistic effect of reducing the MICs and MBCs of and under normoxic and hypoxic conditions due to increasing HO production. Temoporfin increased the biofilm removal of , , and under normoxic condition, and it reduced the antibiotic resistance-related genes expression of MRSA. The combination of temoporfin with ampicillin or chlorhexidine significantly enhanced the bactericidal effect on MRSA. This study provides a potential application of temoporfin on the clinical side against oral pathogens and the prevention of oral diseases.
5,10,15,20-四（3-羟苯基）二氢卟吩（替莫泊芬）是一种用于光动力疗法治疗口腔癌和牙周疾病的光敏剂。本研究测定了替莫泊芬的最低抑菌浓度（MIC）和最低杀菌浓度（MBC）。此外，还测定了在缺氧或常氧条件下碘化钾（KI）或抗菌剂与口腔病原体的联合作用。我们还评估了生物膜清除效果，并检测了耐甲氧西林金黄色葡萄球菌（MRSA）的抗生素耐药相关基因和生物膜形成相关基因的表达。结果表明，替莫泊芬与KI联合使用在常氧和缺氧条件下具有协同作用，可降低MIC和MBC，这是由于增加了羟基自由基（HO）的产生。替莫泊芬在常氧条件下增加了对牙龈卟啉单胞菌、具核梭杆菌和中间普氏菌的生物膜清除作用，并且降低了MRSA的抗生素耐药相关基因表达。替莫泊芬与氨苄西林或氯己定联合使用显著增强对MRSA的杀菌作用。本研究为替莫泊芬在临床上对抗口腔病原体和预防口腔疾病的潜在应用提供了依据。
