Lourenco Nogueira Christiane, Boegel Scott Joseph, Shukla Manish, Ngo William, Jones Lyndon, Aucoin Marc G
Department of Chemical Engineering, University of Waterloo, Waterloo, ON N2L 3G1, Canada.
Centre for Ocular Research & Education (CORE), School of Optometry & Vision Science, University of Waterloo, Waterloo, ON N2L 3G1, Canada.
Pathogens. 2022 Apr 15;11(4):472. doi: 10.3390/pathogens11040472.
Background: Given that reports have suggested SARS-CoV-2 can be transmitted via conjunctiva, the ability of contact lens (CL) care products to reduce the infectiousness of two seasonal human coronavirus (HCoV) (HCoV-229E and HCoV-OC43) surrogates for SARS-CoV-2 was investigated. Methods: Biotrue and Boston Simplus (Bausch&Lomb), OPTI-FREE Puremoist and Clear Care (Alcon), and cleadew and cleadew GP (Ophtecs) were tested. Their ability to inactivate HCoV was evaluated using contact times of 4 and 6 h as well as 1% and 10% of virus inoculum. Results: Non-oxidative systems (Biotrue, Boston Simplus, and OPTI-FREE) did not exhibit a significant log10 reduction compared to controls for the two viral strains for either incubation time (all p > 0.05) when 10% tests were performed. For the 1% test, while Boston Simplus and OPTI-FREE exhibited a significant log10 reduction of both HCoV-229E (after 6 h) and HCoV-OC43 (after either 4 or 6 h incubation), those products showed less than 1 log10 reduction of the two infectious viruses. Oxidative systems based on hydrogen peroxide or povidone-iodine showed a significant log10 reduction compared with the controls for both HCoV-229E and HCoV-OC43 in all tested conditions (all p < 0.01). Clear Care led to virus inactivation to below the limit of quantification for tests performed with 1% of inoculum after 6 h incubation, while cleadew and cleadew GP led to inactivation of the two viruses to below the limit of quantification in all tested conditions. Conclusion: Oxidative CL disinfection systems showed significant virucidal activity against HCoV-229E and HCoV-OC43, while non-oxidative systems showed minimal ability to inactivate the HCoV species examined.
鉴于有报道称严重急性呼吸综合征冠状病毒2（SARS-CoV-2）可通过结膜传播，因此对隐形眼镜护理产品降低两种季节性人类冠状病毒（HCoV）（HCoV-229E和HCoV-OC43）作为SARS-CoV-2替代物的传染性的能力进行了研究。方法：对博士伦的倍明视和波士顿舒视氧、爱尔康的傲滴恒润和清透护理液以及欧普泰克的可丽爱和可丽爱GP进行了测试。使用4小时和6小时的接触时间以及1%和10%的病毒接种量评估它们灭活HCoV的能力。结果：在进行10%测试时，对于两种病毒株，非氧化系统（倍明视、波士顿舒视氧和傲滴恒润）在任何孵育时间下与对照相比均未显示出显著的log10减少（所有p>0.05）。对于1%测试，虽然波士顿舒视氧和傲滴恒润对HCoV-229E（6小时后）和HCoV-OC43（4或6小时孵育后）均显示出显著的log10减少，但这些产品对两种感染性病毒的减少量均小于1个log10。基于过氧化氢或聚维酮碘的氧化系统在所有测试条件下与对照相比，对HCoV-229E和HCoV-OC43均显示出显著的log10减少（所有p<0.01）。清透护理液在孵育6小时后，对于用1%接种量进行的测试，导致病毒灭活至低于定量限，而可丽爱和可丽爱GP在所有测试条件下均导致两种病毒灭活至低于定量限。结论：氧化型隐形眼镜消毒系统对HCoV-229E和HCoV-OC43显示出显著的杀病毒活性，而非氧化系统对所检测的HCoV种类的灭活能力极小。
