L.的抗性质：真菌生长、生物膜形成及抗真菌耐药性的增强
Anti- Properties of L.: Enhancement of Fungal Growth, Biofilm Production and Antifungal Resistance.
作者信息
de Lima Luciene Ferreira, Andrade-Pinheiro Jacqueline Cosmo, Freitas Maria Audilene, da Silva Adriely Idalina, Fonseca Victor Juno Alencar, da Silva Taís Gusmão, da Silva Josefa Carolaine Pereira, de Lima Rosilaine Honorato, Sales Débora Lima, Neves Rejane Pereira, de Brito Edy Sousa, Ribeiro Paulo Riceli Vasconcelos, Canuto Kirley Marques, Coutinho Henrique Douglas Melo, Siyadatpanah Abolghasem, Kim Bonglee, Morais-Braga Maria Flaviana Bezerra
机构信息
Laboratory of Applied Mycology of Cariri (LMAC), University Regional of Cariri (URCA), Crato 63105-010, Brazil.
Bioassay Laboratory (Labio), Federal University of Cariri (UFCA), Brejo Santo 63260-000, Brazil.
出版信息
Pharmaceutics. 2022 Mar 24;14(4):698. doi: 10.3390/pharmaceutics14040698.
(1) Background: is a genus of yeasts with notable pathogenicity and significant ability to develop antimicrobial resistance. L., a medicinal plant that is traditionally used due to its antimicrobial properties, has demonstrated significant antifungal activity. Therefore, this study investigated the chemical composition and anti- effects of aqueous (AELG) and hydroethanolic (HELG) extracts obtained from the leaves of this plant. (2) Methods: The extracts were chemically characterized by UPLC-QTOF-MS/MS, and their anti- activities were investigated by analyzing cell viability, biofilm production, morphological transition, and enhancement of antifungal resistance. (3) Results: The UPLC-QTOF-MS/MS analysis revealed the presence of twenty-one compounds in both AELG and HELG, highlighting the predominance of flavonoids. The combination of the extracts with fluconazole significantly reduced its IC values against INCQS 40006, INCQS 40042, and URM 4262 strains, indicating enhanced antifungal activity. About biofilm production, significant inhibition was observed only for the AELG-treated URM 4262 strain in comparison with the untreated control. Accordingly, this extract showed more significant inhibitory effects on the morphological transition of the INCQS 40006 and URM 4387 strains of (4) Conclusions: L. presents promising antifungal effects, that may be potentially linked to the combined activity of chemical constituents identified in its extracts.
(1) 背景： 是一类具有显著致病性且极易产生抗微生物耐药性的酵母。 ，一种因其抗菌特性而被传统使用的药用植物，已显示出显著的抗真菌活性。因此，本研究调查了从该植物叶片中获得的水提取物（AELG）和氢乙醇提取物（HELG）的化学成分和抗 作用。(2) 方法：通过超高效液相色谱-四极杆飞行时间串联质谱（UPLC-QTOF-MS/MS）对提取物进行化学表征，并通过分析细胞活力、生物膜形成、形态转变和抗真菌耐药性增强来研究其抗 活性。(3) 结果：UPLC-QTOF-MS/MS分析显示AELG和HELG中均存在21种化合物，突出了黄酮类化合物的优势。提取物与氟康唑联合使用显著降低了其对 INCQS 40006、 INCQS 40042和 URM 4262菌株的IC值，表明抗真菌活性增强。关于生物膜形成，与未处理的对照相比，仅观察到AELG处理的 URM 4262菌株有显著抑制作用。因此，该提取物对 INCQS 40006和URM 4387菌株的形态转变显示出更显著的抑制作用。(4) 结论： 显示出有前景的抗真菌作用，这可能与其提取物中鉴定出的化学成分的联合活性潜在相关。
需注意，原文中部分拉丁学名未完整给出，翻译时保留了原样。
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