Nefedova Ekaterina, Shkil Nikolay, Luna Vazquez-Gomez Roberto, Garibo Diana, Pestryakov Alexey, Bogdanchikova Nina
Siberian Federal Scientific Centre of Agro-BioTechnologies of the Russian Academy of Sciences, Novosibirsk 630501, Russia.
Escuela de Ciencias de la Salud, Universidad Autónoma de Baja California, Ensenada 22890, B.C., Mexico.
Pharmaceutics. 2022 Mar 31;14(4):763. doi: 10.3390/pharmaceutics14040763.
The present work presents translational research with application of AgNPs targeting the global drug resistance problem. In vivo fieldwork was carried out with 400 breeding farm cows sick with a serous mastitis. Ex vivo results revealed that after cow treatment with Lactobay (a mixture of antibiotic drugs) the susceptibility to 31 antibiotics of isolates from cow breast secretion decreased by 25%, while after treatment with Argovit-C silver nanoparticles susceptibility increased by 11%. The portion of isolates with an efflux effect leading to elimination of antibiotics from after Lactobay-treatment resulted in a 15% increase, while Argovit-C-treatment led to a 17.5% decrease. The obtained results showed that mastitis treatments with Argovit-C AgNPs can partially restore the activity of antibiotics towards and shorten the duration of mastitis treatment by 33%.
本研究展示了针对全球耐药性问题应用银纳米颗粒的转化研究。对400头患有浆液性乳腺炎的养殖场奶牛进行了实地活体研究。体外研究结果显示，用乳酸乳球菌素（一种抗生素药物混合物）治疗奶牛后，从奶牛乳腺分泌物中分离出的菌株对31种抗生素的敏感性降低了25%，而用阿戈维特-C银纳米颗粒治疗后，敏感性提高了11%。乳酸乳球菌素治疗后，具有外排作用导致抗生素从分泌物中消除的分离株比例增加了15%，而阿戈维特-C治疗导致该比例下降了17.5%。所得结果表明，用阿戈维特-C银纳米颗粒治疗乳腺炎可部分恢复抗生素对其的活性，并将乳腺炎治疗时间缩短33%。
