Kiang Nancy Y, Swingley Wesley D, Gautam Dikshyant, Broddrick Jared T, Repeta Daniel J, Stolz John F, Blankenship Robert E, Wolf Benjamin M, Detweiler Angela M, Miller Kathy Ann, Schladweiler Jacob J, Lindeman Ron, Parenteau Mary N
NASA Goddard Institute for Space Studies, New York, NY 10025, USA.
Virtual Planetary Laboratory, Nexus for Exoplanet System Science, NASA Astrobiology Program, USA.
Microorganisms. 2022 Apr 14;10(4):819. doi: 10.3390/microorganisms10040819.
We have isolated a chlorophyll--containing cyanobacterium from the intertidal field site at Moss Beach, on the coast of Central California, USA, where Manning and Strain (1943) originally discovered this far-red chlorophyll. Here, we present the cyanobacterium's environmental description, culturing procedure, pigment composition, ultrastructure, and full genome sequence. Among cultures of far-red cyanobacteria obtained from red algae from the same site, this strain was an epiphyte on a brown macroalgae. Its Q absorbance peak is centered at 704-705 nm, the shortest wavelength observed thus far among the various known strains. Its Chl /Chl ratio was 0.01, with Chl accounting for 99% of the total Chl and Chl mass. TEM imagery indicates the absence of phycobilisomes, corroborated by both pigment spectra and genome analysis. The Moss Beach strain codes for only a single set of genes for producing allophycocyanin. Genomic sequencing yielded a 7.25 Mbp circular chromosome and 10 circular plasmids ranging from 16 kbp to 394 kbp. We have determined that this strain shares high similarity with strain S15, an epiphyte of red algae, while its distinct gene complement and ecological niche suggest that this strain could be the closest known relative to the original Chl source of Manning and Strain (1943). The Moss Beach strain is designated sp. () strain Moss Beach.
我们从美国加利福尼亚州中部海岸莫斯海滩的潮间带场地分离出了一种含叶绿素的蓝细菌，曼宁和斯特林（1943年）最初就是在那里发现了这种远红光叶绿素。在此，我们展示了该蓝细菌的环境描述、培养过程、色素组成、超微结构和全基因组序列。在从同一地点的红藻中获得的远红光蓝细菌培养物中，该菌株是一种附着在棕色大型藻类上的附生植物。其Q吸收峰集中在704 - 705纳米，这是迄今为止在各种已知菌株中观察到的最短波长。其叶绿素/叶绿素比率为0.01，叶绿素占总叶绿素和叶绿素质量的99%。透射电子显微镜图像显示不存在藻胆体，色素光谱和基因组分析均证实了这一点。莫斯海滩菌株仅编码一组用于产生别藻蓝蛋白的基因。基因组测序产生了一条725万碱基对的环状染色体和10条环状质粒，大小从16千碱基对到394千碱基对不等。我们已经确定该菌株与红藻附生植物S15菌株具有高度相似性，而其独特的基因组成和生态位表明，该菌株可能是与曼宁和斯特林（1943年）最初的叶绿素来源最接近的已知亲属。莫斯海滩菌株被命名为sp. () 菌株莫斯海滩。
