ERG 和行为性 CFF 在光损伤白化大鼠中的变化。

ERG and Behavioral CFF in Light-Damaged Albino Rats.

机构信息

Department of Optometry and Vision Sciences, University of Alabama at Birmingham, 1720 2nd Avenue South, Birmingham, AL 35294, USA.

Department of Neurobiology, University of Alabama at Birmingham, 1720 2nd Avenue South, Birmingham, AL 35294, USA.

出版信息

Int J Mol Sci. 2022 Apr 8;23(8):4127. doi: 10.3390/ijms23084127.

DOI:10.3390/ijms23084127

PMID:35456959

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC9027716/

Abstract

The full-field ERG is useful for index rod- or cone-mediated retinal function in rodent models of retinal degeneration. However, the relationship between the ERG response amplitudes and visually guided behavior, such as flicker detection, is not well understood. A comparison of ERG to behavioral responses in a light-damage model of retinal degeneration allows us to better understand the functional implications of electrophysiological changes. Flicker-ERG and behavioral responses to flicker were used to determine critical flicker frequency (CFF) under scotopic and photopic conditions before and up to 90 d after a 10-day period of low-intensity light damage. Dark- and light-adapted ERG flash responses were significantly reduced after light damage. The a-wave was permanently reduced, while the b-wave amplitude recovered over three weeks after light damage. There was a small, but significant dip in scotopic ERG CFF. Photopic behavioral CFF was slightly lower following light damage. The recovery of the b-wave amplitude and flicker sensitivity demonstrates the plasticity of retinal circuits following photopic injury.

摘要

全视野视网膜电图（ERG）对于评估视网膜变性的啮齿动物模型中的 rod-或 cone-介导的视网膜功能非常有用。然而，ERG 反应幅度与视觉引导行为（如闪烁检测）之间的关系尚不清楚。在光损伤视网膜变性模型中，将 ERG 与行为反应进行比较，可以帮助我们更好地理解电生理变化的功能意义。在 10 天低强度光损伤后，在暗适应和明适应条件下，使用闪烁 ERG 和对闪烁的行为反应来确定临界闪烁频率（CFF）。光损伤后，暗适应和明适应 ERG 闪光反应明显降低。a 波永久降低，而 b 波幅度在光损伤后三周内恢复。暗适应 ERG 的 CFF 略有下降，但有统计学意义。光损伤后，明适应行为 CFF 略低。b 波幅度和闪烁敏感性的恢复表明，光损伤后视网膜回路具有可塑性。