Meng Shuaishuai, Wang Li, Ji Xixi, Yu Jie, Ma Xing, Zhang Jiaheng, Zhao Weiwei, Ji Hongjun, Li Mingyu, Feng Huanhuan
Sauvage Laboratory for Smart Materials, Flexible Printed Electronic Technology Center, School of Materials Science and Engineering, Harbin Institute of Technology (Shenzhen), Shenzhen 518055, China.
School of Materials Science and Engineering, Harbin Institute of Technology (Shenzhen), Shenzhen 518055, China.
Nanomaterials (Basel). 2022 Apr 12;12(8):1327. doi: 10.3390/nano12081327.
Graphene has been considered as an excellent biochemical sensors' substrate material because of its excellent physical and chemical properties. Most of these sensors have employed enzymes, antibodies, antigens, and other biomolecules with corresponding recognition ability as recognition elements, to convert chemical signals into electrical signals. However, oxidoreductase enzymes that grow on graphene surfaces are affected significantly by the environment and are easily inactivated, which hinders the further improvement of detection sensitivity and robusticity. A gold-boosted graphene sensor was fabricated by the in situ electrochemical deposition of inorganic gold nanoparticles on vertical graphene nanosheets. This approach solves the instability of biological enzymes and improves the detection performance of graphene-based sensors. The uric acid sensitivity of the gold-boosted electrode was 6230 µA mM cm, which is 6 times higher than the original graphene electrode. A 7 h GNSs/CC electrode showed an impressive detection performance for ascorbic acid, dopamine, and uric acid, simultaneously. Moreover, it exhibited a reliable detection performance in human serum in terms of uric acid. The possible reason could be that the vertical aliened graphene nanosheet acts as a reaction active spot. This 3D graphene-nanosheet-based doping approach can be applied to a wide variety of inorganic catalytic materials to enhance their performance and improve their durability in aspects such as single-atom catalysis and integration of multiple catalytic properties.
由于其优异的物理和化学性质，石墨烯被认为是一种出色的生化传感器基底材料。这些传感器大多采用具有相应识别能力的酶、抗体、抗原和其他生物分子作为识别元件，将化学信号转化为电信号。然而，生长在石墨烯表面的氧化还原酶受环境影响显著，且容易失活，这阻碍了检测灵敏度和稳定性的进一步提高。通过在垂直石墨烯纳米片上原位电化学沉积无机金纳米颗粒制备了一种金增强石墨烯传感器。这种方法解决了生物酶的不稳定性问题，提高了基于石墨烯的传感器的检测性能。金增强电极对尿酸的灵敏度为6230 µA mM cm，比原始石墨烯电极高6倍。7 h的石墨烯纳米片/碳布电极对抗坏血酸、多巴胺和尿酸同时表现出令人印象深刻的检测性能。此外，它在人血清中对尿酸表现出可靠的检测性能。可能的原因是垂直排列的石墨烯纳米片充当了反应活性位点。这种基于三维石墨烯纳米片的掺杂方法可应用于多种无机催化材料，以提高其性能，并在单原子催化和多种催化性能集成等方面提高其耐久性。
