Lyu Zhaoyuan, Ding Shichao, Wang Maoyu, Pan Xiaoqing, Feng Zhenxing, Tian Hangyu, Zhu Chengzhou, Du Dan, Lin Yuehe
School of Mechanical and Materials Engineering, Washington State University, Pullman, WA, 99164, USA.
School of Chemical, Biological, and Environmental Engineering, Oregon State University, Corvallis, OR, 97331, USA.
Nanomicro Lett. 2021 Jun 19;13(1):146. doi: 10.1007/s40820-021-00661-z.
Fe-based single-atomic site catalysts (SASCs), with the natural metalloproteases-like active site structure, have attracted widespread attention in biocatalysis and biosensing. Precisely, controlling the isolated single-atom Fe-N-C active site structure is crucial to improve the SASCs' performance. In this work, we use a facile ion-imprinting method (IIM) to synthesize isolated Fe-N-C single-atomic site catalysts (IIM-Fe-SASC). With this method, the ion-imprinting process can precisely control ion at the atomic level and form numerous well-defined single-atomic Fe-N-C sites. The IIM-Fe-SASC shows better peroxidase-like activities than that of non-imprinted references. Due to its excellent properties, IIM-Fe-SASC is an ideal nanoprobe used in the colorimetric biosensing of hydrogen peroxide (HO). Using IIM-Fe-SASC as the nanoprobe, in situ detection of HO generated from MDA-MB-231 cells has been successfully demonstrated with satisfactory sensitivity and specificity. This work opens a novel and easy route in designing advanced SASC and provides a sensitive tool for intracellular HO detection.
具有天然类金属蛋白酶活性位点结构的铁基单原子位点催化剂（SASC）在生物催化和生物传感领域引起了广泛关注。确切地说，控制孤立的单原子铁 - 氮 - 碳（Fe-N-C）活性位点结构对于提高SASC的性能至关重要。在这项工作中，我们使用一种简便的离子印迹方法（IIM）合成孤立的Fe-N-C单原子位点催化剂（IIM-Fe-SASC）。通过这种方法，离子印迹过程可以在原子水平上精确控制离子，并形成大量定义明确的单原子Fe-N-C位点。IIM-Fe-SASC表现出比非印迹对照更好的过氧化物酶样活性。由于其优异的性能，IIM-Fe-SASC是用于过氧化氢（HO）比色生物传感的理想纳米探针。使用IIM-Fe-SASC作为纳米探针，已成功证明对MDA-MB-231细胞产生的HO进行原位检测，具有令人满意的数据敏感性和特异性。这项工作为设计先进的SASC开辟了一条新颖且简便的途径，并为细胞内HO检测提供了一种灵敏的工具。
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