Wang Zhenyu, Le Xiehui, Cao Xuesong, Wang Chuanxi, Chen Feiran, Wang Jing, Feng Yan, Yue Le, Xing Baoshan
School of Environment and Civil Engineering, Institute of Environmental Processes and Pollution Control, Jiangnan University, Wuxi 214122, China.
Jiangsu Engineering Laboratory for Biomass Energy and Carbon Reduction Technology, Wuxi 214122, China.
Nanomaterials (Basel). 2022 Apr 13;12(8):1341. doi: 10.3390/nano12081341.
Flavonoids contribute to fruit sensorial and nutritional quality. They are also highly beneficial for human health and can effectively prevent several chronic diseases. There is increasing interest in developing alternative food sources rich in flavonoids, and nano-enabled agriculture provides the prospect for solving this action. In this study, triiron tetrairon phosphate (Fe(PO)) nanomaterials (NMs) were synthesized and amended in soils to enhance flavonoids accumulation in tomato fruits. 50 mg kg of Fe(PO) NMs was the optimal dose based on its outstanding performance on promoting tomato fruit flavonoids accumulation. After entering tomato roots, Fe(PO) NMs promoted auxin (IAA) level by 70.75 and 164.21% over Fe-EDTA and control, and then up-regulated the expression of genes related to PM H ATPase, leading to root proton ef-flux at 5.87 pmol cm s and rhizosphere acidification. More Mg, Fe, and Mn were thus taken up into plants. Subsequently, photosynthate was synthesized, and transported into fruits more rapidly to increase flavonoid synthesis potential. The metabolomic and transcriptomic profile in fruits further revealed that Fe(PO) NMs regulated sucrose metabolism, shi-kimic acid pathway, phenylalanine synthesis, and finally enhanced flavonoid biosynthesis. This study implies the potential of NMs to improve fruit quality by enhancing flavonoids synthesis and accumulation.
类黄酮有助于果实的感官和营养品质。它们对人体健康也非常有益，能够有效预防多种慢性疾病。人们对开发富含类黄酮的替代食物来源的兴趣与日俱增，而纳米农业为解决这一问题提供了前景。在本研究中，合成了磷酸三铁四铁（Fe(PO)）纳米材料并将其施用于土壤中，以提高番茄果实中类黄酮的积累。基于其在促进番茄果实类黄酮积累方面的出色表现，50 mg kg的Fe(PO)纳米材料是最佳剂量。进入番茄根系后，与Fe-EDTA和对照相比，Fe(PO)纳米材料使生长素（IAA）水平分别提高了70.75%和164.21%，随后上调了与质膜H⁺-ATP酶相关基因的表达，导致根质子外流速率达到5.87 pmol cm⁻² s⁻¹ ，并使根际酸化。因此，更多的镁、铁和锰被植物吸收。随后，光合产物得以合成，并更快地运输到果实中，以增加类黄酮的合成潜力。果实的代谢组学和转录组学分析进一步表明，Fe(PO)纳米材料调节了蔗糖代谢、莽草酸途径、苯丙氨酸合成，最终增强了类黄酮的生物合成。本研究表明纳米材料具有通过增强类黄酮合成和积累来改善果实品质的潜力。
