Sayfutdinova Adeliya, Samofalova Irina, Barkov Artem, Cherednichenko Kirill, Rimashevskiy Denis, Vinokurov Vladimir
Department of Physical and Colloidal Chemistry, Gubkin University, 119991 Moscow, Russia.
Department of Traumatology and Orthopedics, RUDN University, 117198 Moscow, Russia.
Polymers (Basel). 2022 Apr 8;14(8):1519. doi: 10.3390/polym14081519.
The current environmental problems require the use of low-energy, environmentally friendly methods and nature-like technologies for the creation of materials. In this work, we aim to study the possibility of the direct biotransformation of fibrillar cellulose by fungi through obtaining a cellulose/mycelium-based biocomposite. The cellulose micro- and nanofibrils were used as the main carbon sources in the solid-phase cultivation of basidiomycete . The cellulose fibrils in this process act as a template for growing mycelium with the formation of well-developed net structure. The biotransformation dynamics of cellulose fibrils were studied with the help of scanning electron microscopy. The appearance of nitrogen in the structure of formed fibers was revealed by elemental analysis and FTIR-spectroscopy. The fibers diameters were estimated based on micrograph analysis and the laser diffraction method. It was shown that the diameter of cellulose fibrils can be tuned by fungi through obtaining cellulose-based mycelium fibers with a narrower diameter-size distribution as compared to the pristine cellulose fibrils. The morphology of the resulting mycelium differed when the micro or nanofibrils were used as a substrate.
当前的环境问题要求使用低能耗、环保的方法以及类似自然的技术来制造材料。在这项工作中，我们旨在通过获得基于纤维素/菌丝体的生物复合材料，研究真菌对纤维状纤维素进行直接生物转化的可能性。纤维素微纤丝和纳米纤丝被用作担子菌固相培养中的主要碳源。在此过程中，纤维素纤维充当菌丝体生长的模板，形成发育良好的网络结构。借助扫描电子显微镜研究了纤维素纤维的生物转化动力学。通过元素分析和傅里叶变换红外光谱（FTIR）揭示了所形成纤维结构中氮的存在。基于显微照片分析和激光衍射法估算了纤维直径。结果表明，与原始纤维素纤维相比，真菌可以通过获得直径尺寸分布更窄的基于纤维素的菌丝体纤维来调节纤维素纤维的直径。当使用微纤丝或纳米纤丝作为底物时，所得菌丝体的形态有所不同。
