Piletsky Stanislav S, Garcia Cruz Alvaro, Piletska Elena, Piletsky Sergey A, Aboagye Eric O, Spivey Alan C
Department of Chemistry, Molecular Sciences Research Hub, White City Campus, Imperial College London, London W12 0BZ, UK.
School of Chemistry, College of Science and Engineering, University of Leicester, Leicester LE1 7RH, UK.
Polymers (Basel). 2022 Apr 14;14(8):1595. doi: 10.3390/polym14081595.
Current state-of-the-art techniques for the solid phase synthesis of molecularly imprinted polymer (MIP) nanoparticles typically rely on amino silanes for the immobilisation of template molecules prior to polymerisation. An investigation into commonly used amino silanes identified a number of problematic side reactions which negatively affect the purity and affinity of these polymers. Iodo silanes are presented as a superior alternative in a case study describing the synthesis of MIPs against epitopes of a common cancer biomarker, epidermal growth factor receptor (EGFR). The proposed iodo silane outperformed the amino silane by all metrics tested, showing high purity and specificity, and nanomolar affinity for the target peptide.
目前，用于分子印迹聚合物（MIP）纳米颗粒固相合成的先进技术通常依赖于氨基硅烷在聚合前固定模板分子。对常用氨基硅烷的研究发现了一些有问题的副反应，这些副反应会对这些聚合物的纯度和亲和力产生负面影响。在一项描述针对常见癌症生物标志物表皮生长因子受体（EGFR）表位的MIP合成的案例研究中，碘代硅烷被提出作为一种更好的替代物。在所测试的所有指标上，所提出的碘代硅烷都优于氨基硅烷，表现出高纯度和特异性，以及对目标肽的纳摩尔亲和力。
