National Institute of Animal Health, National Agriculture and Food Research Organization, 3-1-5 Kannondai, Tsukuba 305-0856, Japan.
United Graduate School of Veterinary Sciences, Gifu University, 1-1 Yanagido, Gifu 501-1193, Japan.
Viruses. 2022 Mar 29;14(4):716. doi: 10.3390/v14040716.
The complete nucleotide sequence of the S1 glycoprotein gene of the Japanese infectious bronchitis virus (IBV) strains was determined and genetically analyzed. A total of 61 Japanese IBV strains were classified into seven genotypes, namely GI-1, 3, 7, 13, 18, 19, and GVI-1 using the classification scheme that was proposed by Valastro et al, with three exceptions. These genotypes practically corresponded to those defined in Japan, namely Mass, Gray, JP-II, 4/91, JP-I, JP-III, and JP-IV, which have been identified through their partial nucleotide sequences containing hypervariable regions 1 and 2. In addition, three exceptive strains were considered to be derived from recombination within the S1 gene of IBV strains G1-13 and GI-19. By analyzing the amino acid polymorphism of the S1 glycoprotein among Japanese genotypes, a diversity was observed based on the genotype-specific amino acid residue, the proteolytic cleavage motif at the S1/S2 cleavage site, and the position of the potential N-glycosylation sites.
测定并分析了日本传染性支气管炎病毒（IBV）株 S1 糖蛋白基因的完整核苷酸序列。利用 Valastro 等人提出的分类方案，将 61 株日本 IBV 株分为 7 个基因型，即 GI-1、3、7、13、18、19 和 GVI-1，有 3 个例外。这些基因型与通过其包含高变区 1 和 2 的部分核苷酸序列鉴定的日本的 Mass、Gray、JP-II、4/91、JP-I、JP-III 和 JP-IV 基本对应。此外，3 个例外株被认为是 IBV 株 G1-13 和 GI-19 的 S1 基因内重组的结果。通过分析日本基因型 S1 糖蛋白的氨基酸多态性，观察到基于基因型特异性氨基酸残基、S1/S2 裂解位点的蛋白水解裂解模体以及潜在 N-糖基化位点的多样性。
