Department of Scienze della Vita e dell'Ambiente, University of Cagliari, 09124 Cagliari, Italy.
Biopharmaceutics and Pharmacokinetics Unit, Institute for Nanoscience and Nanotechnology, University of Barcelona, 08007 Barcelona, Spain.
Molecules. 2022 Apr 8;27(8):2423. doi: 10.3390/molecules27082423.
Culture of plant cells or tissues is a scalable, sustainable, and environmentally friendly approach to obtain extracts and secondary metabolites of uniform quality that can be continuously supplied in controlled conditions, independent of geographical and seasonal variations, environmental factors, and negative biological influences. In addition, tissues and cells can be extracted/obtained from the by-products of other industrial cultivations such as that of Miller (), which is largely cultivated for the collection of flowers. Given that, an extract rich in rosmarinic acid was biotechnologically produced starting from cell suspension of , which was then loaded in hyalurosomes, special phospholipid vesicles enriched with sodium hyaluronate, which in turn are capable of both immobilizing and stabilizing the system. These vesicles have demonstrated to be good candidates for skin delivery as their high viscosity favors their residence at the application site, thus promoting their interaction with the skin components. The main physico-chemical and technological characteristics of vesicles (i.e., mean diameter, polydispersity index, zeta potential and entrapment efficiency of extract in vesicles) were measured along with their biological properties in vitro: biocompatibility against fibroblasts and ability to protect the cells from oxidative stress induced by hydrogen peroxide. Overall, preliminary results disclosed the promising properties of obtained formulations to be used for the treatment of skin diseases associated with oxidative stress and inflammation.
植物细胞或组织的培养是一种可扩展、可持续且环保的方法，可以获得具有均匀质量的提取物和次生代谢产物，这些产物可以在控制条件下持续供应，不受地理和季节变化、环境因素以及负面生物影响的影响。此外，组织和细胞可以从其他工业培养物（如 Miller（米勒）的副产物中提取/获得），Miller 主要用于采集花朵。鉴于此，从细胞悬浮液中生物技术生产了富含迷迭香酸的提取物，然后将其加载到透明质酸囊中，透明质酸囊是一种富含透明质酸钠的特殊磷脂囊，能够固定和稳定该系统。这些囊泡已被证明是皮肤传递的良好候选物，因为它们的高粘度有利于它们在应用部位的停留，从而促进它们与皮肤成分的相互作用。测量了囊泡的主要物理化学和技术特性（即囊泡的平均直径、多分散指数、ζ 电位和提取物在囊泡中的包封效率）及其体外生物学特性：对成纤维细胞的生物相容性以及保护细胞免受由过氧化氢诱导的氧化应激的能力。总的来说，初步结果显示了所获得的制剂用于治疗与氧化应激和炎症相关的皮肤疾病的有希望的特性。
