Gismondi Angelo, Canuti Lorena, Grispo Marta, Canini Antonella
Photochem Photobiol. 2014 May-Jun;90(3):702-8. doi: 10.1111/php.12229. Epub 2014 Jan 28.
UV radiations are principal causes of skin cancer and aging. Suntan creams were developed to protect epidermis and derma layers against photodegradation and photooxidation. The addition of antioxidant plant extracts (i.e. essential oil) to sunscreens is habitually performed, to increase their UV protective effects and to contrast pro-radical and cytotoxic compounds present in these solutions. According to these observations, in the present work, the alteration of chemical composition and bioactive properties of Lavandula angustifolia Miller essential oil, exposed to UV light, was investigated. UV induced a significant deterioration of lavender oil biochemical profile. Moreover, the antioxidant activity of this solution, in in vitro tests and directly on B16-F10 melanoma cells, greatly decreased after UV treatment. Our results also showed that essential oil was shielded from UV stress by propolis addition. Even after UV treatment, bee glue highly protected lavender oil secondary metabolites from degradation and also preserved their antiradical properties, both in in vitro antioxidant assays and in cell oxidative damage evaluations. This research proposed propolis as highly efficient UV protective and antiradical additive for sunscreens, cosmetics and alimentary or pharmaceutical products containing plant extracts.
紫外线辐射是皮肤癌和皮肤老化的主要原因。防晒乳的研发旨在保护表皮和真皮层免受光降解和光氧化作用。习惯在防晒霜中添加抗氧化植物提取物（即精油），以增强其紫外线防护效果，并对抗这些溶液中存在的促自由基和细胞毒性化合物。基于这些观察结果，在本研究中，我们对经紫外线照射的薰衣草精油的化学成分和生物活性特性的变化进行了研究。紫外线导致薰衣草油的生化特征显著恶化。此外，在体外试验以及直接作用于B16-F10黑色素瘤细胞时，该溶液经紫外线处理后的抗氧化活性大幅下降。我们的研究结果还表明，添加蜂胶可使精油免受紫外线胁迫。即使经过紫外线处理，在体外抗氧化试验和细胞氧化损伤评估中，蜂胶仍能高度保护薰衣草油的次生代谢产物不被降解，并保留其抗自由基特性。本研究提出，蜂胶可作为一种高效的紫外线防护和抗自由基添加剂，用于防晒霜、化妆品以及含有植物提取物的食品或药品中。
