一种用于鸟氨酸脱羧酶活性放射性测定的离子交换法的可行变体。
A practicable variant of the ion exchange method for the radiometric estimation of ornithine decarboxylase activity.
作者信息
Weber L W
出版信息
Experientia. 1987 Feb 15;43(2):176-8. doi: 10.1007/BF01942841.
PMID:3545892
Abstract
A known ornithine decarboxylase assay working with ion exchange separation of [3H]ornithine and [3H]putrescine has been revised. The assay can be performed in disposable 1.5 ml vessels with a total of four pipetting steps. The separation of enzyme substrate and product, respectively, requires 3 h per 50 samples. The detection limit is about 50 pmoles [3H]putrescine formed.
摘要
一种使用离子交换法分离[3H]鸟氨酸和[3H]腐胺的已知鸟氨酸脱羧酶测定法已得到改进。该测定法可在一次性1.5毫升容器中进行，总共只需四个移液步骤。酶底物和产物的分离，每50个样品分别需要3小时。检测限约为形成的50皮摩尔[3H]腐胺。
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