一种利用液相双抗体系统检测小鼠表皮生长因子的酶免疫测定法。
An enzyme immunoassay for mouse epidermal growth factor utilizing a liquid phase double-antibody system.
作者信息
Kashimata M, Hiramatsu M, Minami N, Minami N
出版信息
Experientia. 1987 Feb 15;43(2):191-2. doi: 10.1007/BF01942847.
PMID:3545895
Abstract
An enzyme immunoassay for mouse epidermal growth factor (EGF) involving a liquid phase double-antibody system was developed. The EGF-beta-galactosidase conjugate prepared was stable for at least 8 months. By this method, EGF was detectable at a concentration as low as 20 pg per tube. The concentrations of EGF in various tissues of mice are also presented.
摘要
开发了一种用于小鼠表皮生长因子（EGF）的酶免疫测定法，该方法涉及液相双抗体系统。所制备的EGF-β-半乳糖苷酶缀合物至少稳定8个月。通过这种方法，每管中低至20 pg浓度的EGF均可被检测到。还给出了小鼠各种组织中EGF的浓度。
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