Perheentupa J, Lakshmanan J, Hoath S B, Beri U, Kim H, Macaso T, Fisher D A
Am J Physiol. 1985 Apr;248(4 Pt 1):E391-6. doi: 10.1152/ajpendo.1985.248.4.E391.
A highly sensitive mouse plasma (serum) epidermal growth factor (EGF) radioimmunoassay was utilized to determine EGF levels in mouse plasma and submandibular gland (SMG) at different ages. Plasma and serum levels were not different. Inferior vena caval blood was used because even at the age of 12 days EGF levels were markedly higher in neck blood. At 2 wk serum EGF levels were 60% of adult female levels. After a temporary decrease, mean EGF concentrations reached adult values at 31 and 60 days in the female but not yet in the male. In adults male serum EGF levels were twofold higher than female levels. This difference is parallel to but much smaller than the sex difference in SMG-EGF. SMG-EGF showed a very different ontogeny. Moreover, no correlation was present in the adults between the SMG and serum EGF concentrations. Testosterone treatment of adult females doubled their serum EGF levels, but the stimulated values were still below the mean of the male levels. The present observed plasma (serum) EGF levels in the mouse are 20-40% of previously reported values, probably because of the absence of EGF contamination from hair (urine) and milk.
采用一种高度灵敏的小鼠血浆（血清）表皮生长因子（EGF）放射免疫分析法，来测定不同年龄小鼠血浆和下颌下腺（SMG）中的EGF水平。血浆和血清水平并无差异。使用下腔静脉血，因为即使在12日龄时，颈部血液中的EGF水平也明显更高。在2周龄时，血清EGF水平为成年雌性水平的60%。经过短暂下降后，雌性小鼠的平均EGF浓度在31天和60天时达到成年值，但雄性小鼠尚未达到。成年雄性血清EGF水平比雌性水平高两倍。这种差异与SMG-EGF中的性别差异平行，但比其小得多。SMG-EGF表现出非常不同的个体发育过程。此外，在成年小鼠中，SMG和血清EGF浓度之间不存在相关性。对成年雌性小鼠进行睾酮处理后，其血清EGF水平翻倍，但刺激后的数值仍低于雄性水平的平均值。目前观察到的小鼠血浆（血清）EGF水平为先前报道值的20%-40%，这可能是由于毛发（尿液）和乳汁中不存在EGF污染。
