Kongkaew Supatinee, Tubtimtong Suowarot, Thavarungkul Panote, Kanatharana Proespichaya, Chang Kah Haw, Abdullah Ahmad Fahmi Lim, Limbut Warakorn
Center of Excellence for Trace Analysis and Biosensors (TAB-CoE), Prince of Songkla University, Hat Yai, Songkhla 90110, Thailand.
Center of Excellence for Innovation in Chemistry, Faculty of Science, Prince of Songkla University, Hat Yai, Songkhla 90110, Thailand.
Sensors (Basel). 2022 Apr 15;22(8):3034. doi: 10.3390/s22083034.
Multichannel graphite electrodes (MGrEs) have been designed and fabricated in this study. A template was cut from an adhesive plastic sheet using a desktop cutting device. The template was placed on a polypropylene substrate, and carbon graphite ink was applied with a squeegee to the template. The size of the auxiliary electrode (AE) as well as the location of the reference electrode (RE) of MGrEs design were investigated. Scanning electron microscopy was used to determine the thickness of the ink on the four working electrodes (WEs), which was 21.9 ± 1.8 µm. Cyclic voltammetry with a redox probe solution was used to assess the precision of the four WEs. The intra-electrode repeatability and inter-electrode reproducibility of the MGrEs production were satisfied by low RSD (<6%). Therefore, the MGrEs is reliable and capable of detecting four replicates of the target analyte in a single analysis. The electrochemical performance of four WEs was investigated and compared to one WE. The sensitivity of the MGrEs was comparable to the sensitivity of a single WE. The MGrEs’ potential applications were investigated by analyzing the nitrite in milk and tap water samples (recoveries values of 97.6 ± 0.4 to 110 ± 2%).
在本研究中设计并制造了多通道石墨电极（MGrEs）。使用桌面切割设备从粘性塑料片上切割出一个模板。将模板放置在聚丙烯基板上，并用刮板将碳石墨油墨施加到模板上。研究了MGrEs设计中辅助电极（AE）的尺寸以及参比电极（RE）的位置。使用扫描电子显微镜来确定四个工作电极（WEs）上油墨的厚度，其为21.9±1.8μm。使用含有氧化还原探针溶液的循环伏安法来评估四个工作电极的精度。MGrEs生产的电极内重复性和电极间再现性通过低相对标准偏差（<6%）得以满足。因此，MGrEs可靠且能够在单次分析中检测目标分析物的四个重复样品。研究了四个工作电极的电化学性能并与一个工作电极进行比较。MGrEs的灵敏度与单个工作电极的灵敏度相当。通过分析牛奶和自来水样品中的亚硝酸盐来研究MGrEs的潜在应用（回收率值为97.6±0.4至110±2%）。
