Section of Anaesthesiology, Department of Clinical Diagnostics and Services, Vetsuisse Faculty, University of Zurich, 8057 Zurich, Switzerland.
Section of Anaesthesiology, Department of Clinical Diagnostics and Services, Vetsuisse Faculty, University of Zurich, 8057 Zurich, Switzerland.
Vet Anaesth Analg. 2022 Jul;49(4):336-343. doi: 10.1016/j.vaa.2021.07.007. Epub 2022 Jan 7.
To measure the effects on microcirculation of medetomidine alone (MED) or combined with vatinoxan (MVX).
Randomized, crossover, blinded, experimental study.
A group of eight healthy purpose-bred Beagle dogs.
Each dog was given 1 mg m MED intramuscularly (IM) or combined with 20 mg m vatinoxan IM (MVX) with a washout period of 7 days. A sidestream dark field (SDF) camera was placed on the buccal mucosa to assess the oral mucosal microcirculation for perfused DeBacker density, proportion of perfused vessels (PPV) (both for all vessels and vessels with a diameter < 20 μm), microvascular flow index (MFI) and heterogeneity index (HI). Videos were recorded at baseline (-5) and 10, 20, 30, 40, 60, 90 and 120 minutes after treatment administration. Linear mixed-effects models were used to assess if microvascular variables were significantly associated with treatment, baseline, and sequence. Results are presented as estimated effect (95% confidence interval), and a p value < 0.05 was considered significant.
The interquartile range for baseline measurements was 91.49%-98.42% for PPV, 2.75-3 for MFI and 0-0.36 for HI. Significant effects of treatment and baseline were found. The estimated effect of MED against MVX was -1.98% (-3.53% to -0.42%) for PPV, -0.33 (-0.43 to -0.22) for MFI and 0.14 (0.05 to 0.22) for HI. There were no significant changes seen for perfused DeBacker density, perfused DeBacker density < 20 μm and PPV < 20 μm between treatments.
These results suggest that MVX had significantly fewer effects on buccal mucosal microcirculation than MED. The SDF camera is a useful research tool to assess the microcirculatory status of heavily sedated dogs.
测量单独使用右美托咪定（MED）或与戊甲溴铵（MVX）联合使用对微循环的影响。
随机、交叉、双盲、实验研究。
一组 8 只健康的专门饲养的比格犬。
每只狗肌肉内注射 1mg/m MED（IM）或联合 IM 注射 20mg/m 戊甲溴铵（MVX），洗脱期为 7 天。放置侧流暗场（SDF）相机于颊黏膜上，以评估口腔黏膜微循环的灌流德贝克密度、灌注血管比例（所有血管和直径 < 20μm 的血管）、微血管血流指数（MFI）和异质性指数（HI）。在治疗后 -5 分钟和 10、20、30、40、60、90 和 120 分钟时记录视频。线性混合效应模型用于评估微血管变量是否与治疗、基线和序列显著相关。结果以估计效应（95%置信区间）表示，p 值<0.05 被认为有统计学意义。
基线测量的四分位距为 PPV 91.49%-98.42%，MFI 2.75-3，HI 0-0.36。发现治疗和基线有显著影响。MED 与 MVX 的估计效应为 PPV -1.98%（-3.53%至-0.42%），MFI -0.33（-0.43 至-0.22），HI 0.14（0.05 至 0.22）。两种治疗方法之间，灌流德贝克密度、<20μm 灌流德贝克密度和<20μm 灌注血管比例均无显著变化。
这些结果表明，MVX 对颊黏膜微循环的影响明显小于 MED。SDF 相机是一种有用的研究工具，可用于评估镇静犬的微循环状态。
