Steblaj Barbara, Joerger Fabiola Binia, Hartnack Sonja, Briganti Angela, Kutter Annette P N
Section of Anaesthesiology, Department of Clinical Diagnostics and Services, Vetsuisse Faculty, University of Zurich, 8057 Zurich, Switzerland.
Section of Epidemiology, Vetsuisse Faculty, University of Zurich, 8057 Zurich, Switzerland.
Animals (Basel). 2025 Jun 17;15(12):1779. doi: 10.3390/ani15121779.
Endotoxemia often leads to microcirculatory derangement. In six sevoflurane anaesthetized Beagle dogs, we investigated the effects of 1 mg/kg of lipopolysaccharide endotoxin intravenous and blood pressure (mean arterial pressure of 65 mmHg versus 85 mmHg) on microcirculation assessed on buccal mucosa using side stream dark field microscopy. Dogs were afterwards resuscitated with fluids and noradrenaline. We investigated dose requirements of noradrenaline with or without dexmedetomidine. Microcirculatory parameters, and markers of sepsis (cardiac output, mixed venous oxygen saturation, carbon dioxide gap, and lactate) were analysed before endotoxemia, after endotoxemia, after a 30 mL/kg of Ringer's acetate fluid bolus, and during noradrenaline +/- dexmedetomidine infusion, after a second fluid bolus, and a second time after vasopressor treatment in a cross-over fashion. Endotoxemia and mean arterial pressure had no statistically significant effect on microcirculation; however, endotoxemia resulted in a decrease in cardiac output. Dexmedetomidine neither improved microcirculation nor reduced noradrenaline requirements.
内毒素血症常导致微循环紊乱。在6只接受七氟醚麻醉的比格犬中，我们研究了静脉注射1mg/kg脂多糖内毒素以及血压（平均动脉压65mmHg与85mmHg）对使用侧流暗视野显微镜在颊黏膜评估的微循环的影响。随后用液体和去甲肾上腺素对犬进行复苏。我们研究了使用或不使用右美托咪定情况下去甲肾上腺素的剂量需求。在内毒素血症前、内毒素血症后、给予30mL/kg醋酸林格液推注后以及在去甲肾上腺素+/-右美托咪定输注期间、第二次液体推注后以及血管升压药治疗后，以交叉方式分析微循环参数以及脓毒症标志物（心输出量、混合静脉血氧饱和度、二氧化碳差值和乳酸）。内毒素血症和平均动脉压对微循环无统计学显著影响；然而，内毒素血症导致心输出量降低。右美托咪定既未改善微循环也未减少去甲肾上腺素需求。
