State Key Laboratory of Organic Geochemistry, Guangdong Provincial Key Laboratory of Environmental Protection and Resources Utilization, Guangzhou Institute of Geochemistry, Chinese Academy of Sciences, Guangzhou 510640, China; CAS Center for Excellence in Deep Earth Science, Guangzhou 510640, China.
State Key Laboratory of Organic Geochemistry, Guangdong Provincial Key Laboratory of Environmental Protection and Resources Utilization, Guangzhou Institute of Geochemistry, Chinese Academy of Sciences, Guangzhou 510640, China; CAS Center for Excellence in Deep Earth Science, Guangzhou 510640, China; University of the Chinese Academy of Sciences, Beijing 100049, China.
Water Res. 2022 Jun 30;218:118464. doi: 10.1016/j.watres.2022.118464. Epub 2022 Apr 14.
Tris(2-chloroethyl) phosphate (TCEP) is of growing concern because of its ubiquitous occurrence, potential toxicity, and persistence in the environment. In this study, two efficient TCEP degradation consortia (AT1 and AT3) were developed and were able to completely hydrolyze TCEP within 20-25 h. Rhizobiales was identified as the key degrader in both consortia, because Rhizobiales-related phosphoesterase genes were enriched by one to two orders of magnitude when the carbon source was changed from acetate to TCEP. In addition, the increase in Rhizobiales abundance was related to the development of TCEP degradation. The isolation of Xanthobacter strains confirmed the efficient TCEP and bis(2-chloroethyl) phosphate (BCEP) degradation of Rhizobiales. The higher abundances of phosphoesterase genes affiliated with Rhizobiales genera (Bradyrhizobium and Ancylobacter), Cytophagales genus (Spirosoma), Sphingobacteriales genus (Pedobacter), and Burkholderia genus (Methylibium), may be related to the faster TCEP degradation in AT3, while the higher abundance of Rhizobiales genus (Hyphomicrobium)-related phosphodiesterase (PDE) genes may contribute to the faster BCEP degradation in AT1. The stepwise hydrolysis of TCEP was likely catalyzed by different bacterial guilds, which was confirmed by the coculture of TCEP- and BCEP-degrading isolates and highlighted the importance of synergistic interactions during TCEP degradation.
磷酸三（2-氯乙基）酯（TCEP）由于其普遍存在、潜在毒性和在环境中的持久性而引起了越来越多的关注。在这项研究中，开发了两种高效的 TCEP 降解菌群（AT1 和 AT3），它们能够在 20-25 小时内完全水解 TCEP。根瘤菌被确定为两种菌群中的关键降解菌，因为当碳源从乙酸盐变为 TCEP 时，根瘤菌相关的磷酸酯酶基因富集了一到两个数量级。此外，根瘤菌丰度的增加与 TCEP 降解的发展有关。Xanthobacter 菌株的分离证实了根瘤菌对 TCEP 和双（2-氯乙基）磷酸酯（BCEP）的高效降解。与 Rhizobiales 属（Bradyrhizobium 和 Ancylobacter）、Cytophagales 属（Spirosoma）、Sphingobacteriales 属（Pedobacter）和 Burkholderia 属（Methylibium）相关的磷酸酯酶基因的丰度较高，可能与 AT3 中更快的 TCEP 降解有关，而 Rhizobiales 属（Hyphomicrobium）相关的磷酸二酯酶（PDE）基因的丰度较高可能有助于 AT1 中更快的 BCEP 降解。TCEP 的逐步水解可能是由不同的细菌类群催化的，这通过共培养 TCEP 和 BCEP 降解分离株得到了证实，并强调了在 TCEP 降解过程中协同相互作用的重要性。
