Student Research Committee, School of Medicine, Kermanshah University of Medical, Sciences, Kermanshah, Iran.
Department of Pediatrics, School of Medicine, Kermanshah University of Medical Sciences, Kermanshah, Iran.
Cytokine. 2022 Jun;154:155889. doi: 10.1016/j.cyto.2022.155889. Epub 2022 Apr 19.
Emerged coronavirus disease 2019 (COVID-19) is a pandemic caused by the severe acute respiratory syndrome coronavirus 2 (SARS-COV-2). Disease severity is associated with elevated levels of proinflammatory cytokines, such as interleukin-6 (IL-6). Genetic polymorphisms in the regulatory regions of cytokine genes may be associated with differential cytokine production in COVID-19 patients. This study aimed to investigate the association between three potentially functional single-nucleotide polymorphisms (SNPs) in the promoter region of IL-6 and the severity of susceptibility to COVID-19 in an Iranian population.
In total, 346 individuals (175 patients with severe COVID-19 and 171 patients with mild COVID-19) were recruited for this cohort study. Genomic DNA was extracted from peripheral blood leukocytes of patients to determine the genotypes of three selected SNPs (rs1800795 (-174 G > C), rs1800796 (-572 G > C), and rs1800797 (-597 G > A)) in the promoter region of the IL-6 gene using polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) method.
There were no significant differences in the genotype or allele distribution of selected SNPs (rs1800795 (-174 G > C), rs1800796 (-572 G > C), and rs1800797 (-597 G > A)) in the promoter region of the IL-6 gene in patients with severe COVID-19 and patients with mild COVID-19.
Our study indicated that these SNPs are not associated with COVID-19 severity in the Kurdish population from Kermanshah, Iran.
新型冠状病毒病 2019（COVID-19）是由严重急性呼吸系统综合征冠状病毒 2（SARS-COV-2）引起的大流行疾病。疾病严重程度与促炎细胞因子（如白细胞介素-6（IL-6））水平升高有关。细胞因子基因调节区的遗传多态性可能与 COVID-19 患者的细胞因子产生存在差异有关。本研究旨在探讨伊朗人群中 IL-6 启动子区三个潜在功能单核苷酸多态性（SNP）与 COVID-19 易感性严重程度的关系。
共纳入 346 名个体（175 名重症 COVID-19 患者和 171 名轻症 COVID-19 患者）进行这项队列研究。从患者外周血白细胞中提取基因组 DNA，采用聚合酶链反应-限制性片段长度多态性（PCR-RFLP）方法检测 IL-6 基因启动子区三个选定 SNP（rs1800795（-174 G>C）、rs1800796（-572 G>C）和 rs1800797（-597 G>A））的基因型。
在重症 COVID-19 患者和轻症 COVID-19 患者中，IL-6 基因启动子区选定 SNP（rs1800795（-174 G>C）、rs1800796（-572 G>C）和 rs1800797（-597 G>A））的基因型或等位基因分布无显著差异。
本研究表明，在伊朗克尔曼沙阿的库尔德人群中，这些 SNP 与 COVID-19 严重程度无关。
