Passos-Silva Ana Maísa, Araújo Adrhyan, Roca Tárcio Peixoto, Queiroz Jackson Alves da Silva, Sgorlon Gabriella, Rampazzo Rita de Cássia Pontello, Salcedo Juan Miguel Villalobos, Zuliani Juliana Pavan, Vieira Deusilene
Laboratório de Virologia Molecular, Fundação Oswaldo Cruz Rondônia - FIOCRUZ/RO, Porto Velho, Rondônia, Brazil.
Programa de Pós-Graduação em Biologia Experimental, Universidade Federal de Rondônia - UNIR/FIOCRUZ/RO, Porto Velho, Rondônia, Brazil.
Biomed Eng Comput Biol. 2025 Apr 30;16:11795972241298786. doi: 10.1177/11795972241298786. eCollection 2025.
The performance and genetic role in host response delineate investigative points of polymorphisms as potential biomarkers in viral infections.
Thus, this research aimed to map biomarkers and risk factors in the severity of COVID-19 in individuals in Western Amazon (n = 243).
Patients aged 40 to 59 years showed an association with clinical progression ( = .003), also evidencing the relationship for individuals >60 years ( < .001), besides the non-vaccination influenced the pathology ( = .023). qPCR for human genotyping of the targets rs2070788, rs4702, rs76635825, rs540856718, rs35803318, rs12979860, and rs16899066, as well as for gene expression of ACE2, HLA-A, HLA-B, IFNL-3/2, IL-6, and TMPRSS2 was used. The rs12979860 (C > T) and rs2070788 (A > G) showed association among the analyzed groups ( < .05) with the allelic and genotypic frequency of rs12979860 ( < 3.84) and evolutionary pointing of rs2070788G allele among infected people, including deaths.
Gene expression showed high levels between the moderate and severe groups, with emphasis on TMPRSS2 and IL-6 genes that performed better. Thus, there is possibly an association regarding the role of the TMPRSS2 gene and rs2070788G, as well as age and IL-6 levels for COVID-19, pointing in parallel to the considerable influence of the vaccine on the SARS-CoV-2 pathway.
多态性在宿主反应中的表现及遗传作用确定了其作为病毒感染潜在生物标志物的研究要点。
因此，本研究旨在确定西亚马逊地区个体（n = 243）中新冠病毒疾病严重程度的生物标志物和风险因素。
40至59岁的患者与临床进展相关（P = 0.003），此外，60岁以上个体也存在这种关系（P < 0.001），并且未接种疫苗会影响病情（P = 0.023）。采用qPCR对目标rs2070788、rs4702、rs76635825、rs540856718、rs35803318、rs12979860和rs16899066进行人类基因分型，同时检测ACE2、HLA - A、HLA - B、IFNL - 3/2、IL - 6和TMPRSS2的基因表达。rs12979860（C>T）和rs2070788（A>G）在分析组中显示出与等位基因和基因型频率的相关性（P < 0.05），rs12979860的χ² < 3.84，并且rs2070788G等位基因在感染者（包括死亡者）中具有进化指向性。
基因表达在中度和重度组之间显示出高水平，尤其TMPRSS2和IL - 6基因表现更为突出。因此，TMPRSS2基因和rs2070788G的作用、年龄以及IL - 6水平与新冠病毒疾病可能存在关联，同时也表明疫苗对严重急性呼吸综合征冠状病毒2途径有显著影响。
