State Key Laboratory of Environment Health (Incubation), Key Laboratory of Environment and Health, Ministry of Education, Key Laboratory of Environment and Health (Wuhan), Ministry of Environmental Protection, School of Public Health, Tongji Medical College, Huazhong University of Science and Technology, Hangkong Road #13, Wuhan, Hubei, 430030, China.
Department of Forensic Medicine, Huazhong University of Science and Technology, Hangkong Road #13, Wuhan, Hubei, 430030, China.
Talanta. 2022 Aug 15;246:123485. doi: 10.1016/j.talanta.2022.123485. Epub 2022 Apr 15.
Coextraction of different groups of analytes is vital for saving sample volumes and simplifying analytical procedures in bioanalysis. Conventionally, coextraction was achieved by using multi-extraction systems with different supported liquid membranes (SLMs). However, the different membrane solvents tended to diffuse into the aqueous solutions and the other SLM to reach distribution equilibrium during extraction process, causing the system instability. In this work, a stable multi-extraction system (integration of liquid-phase microextraction and electromembrane extraction, LPME/EME) based on the identical supported semi-liquid membrane (SsLM) was developed. Principally, the state of distribution equilibrium of the membrane solvent (polypropylene glycol with molecular weight 4000) in SsLM could be reached at the beginning of extraction, which enhanced the coextraction stability. With this multi-extraction system, acidic and basic analytes were simultaneously extracted from practical biological samples. The extraction recoveries of the six model drugs in undiluted urine samples were over 70%. Followed by LC-MS/MS, the limits of quantification (LOQs) were in the range of 5-10 ng mL. The multi-extraction system using the identical SsLM in this study shows promising potential in construction of other stable multi-extraction systems (e.g., LPME/LPME and EME/EME) in the future, which will greatly benefit the group separation of analytes in complicated biological samples.
不同组分析物的共提取对于节省样品体积和简化生物分析中的分析程序至关重要。传统上，通过使用具有不同支撑液膜（SLM）的多萃取系统来实现共提取。然而，不同的膜溶剂在萃取过程中往往会扩散到水溶液中，而其他的 SLM 则会达到分配平衡，从而导致系统不稳定。在这项工作中，开发了一种基于相同支撑半液态膜（SsLM）的稳定多萃取系统（液相微萃取和电膜萃取的集成，LPME/EME）。原则上，在萃取开始时，SsLM 中膜溶剂（分子量为 4000 的聚丙二醇）的分配平衡状态即可达到，从而增强了共提取的稳定性。使用这种多萃取系统，可以从实际生物样品中同时提取酸性和碱性分析物。未稀释尿液样品中六种模型药物的萃取回收率均超过 70%。通过 LC-MS/MS，检测限（LOQs）在 5-10ng/mL 范围内。本研究中使用相同 SsLM 的多萃取系统在未来构建其他稳定的多萃取系统（例如 LPME/LPME 和 EME/EME）方面显示出有前途的潜力，这将极大地有利于复杂生物样品中分析物的分组分离。
