School of Pharmacy, Jiamusi University, Jiamusi 154007, China.
The College of Traditional Chinese Medicine, Guangdong Pharmaceutical University, Guangzhou 510006, China.
Biomed Pharmacother. 2022 Jun;150:112990. doi: 10.1016/j.biopha.2022.112990. Epub 2022 Apr 21.
As a traditional Chinese medicine, Dalbergia tsoi Merr.et Chun (JZX) has been used for the treatment of wounds since ancient times. However, the active compounds and molecular mechanisms of JZX in the acceleration of wound healing are still unknown. Herein, we explored the main active compounds and key molecular mechanisms by which JZX accelerates wound healing. The ethanol extract of JZX was subjected to UPLC-Q-Orbitrap HRMS analysis to identify the main compounds. The pharmacological effect of JZX on wound healing was evaluated using a mouse excision wound model. Network pharmacology was utilized to predict the effective compounds and related signal transduction pathways of JZX that were involved in accelerating wound healing. The predicted key signaling pathways were then validated by immunohistochemical analysis. Interactions between the active compounds and therapeutic targets were confirmed by molecular docking analysis. JZX accelerated wound healing, improved tissue quality, and inhibited inflammation and oxidative stress. Moreover, our results suggested that the active components of JZX, such as butin, eriodyctiol, and formononetin, are the key compounds that facilitate wound treatment. Our studies also indicated that JZX accelerated wound healing by regulating the PI3K/Akt signaling pathway and inducing the expression of TGF-β1, FGF2, VEGFA, ECM1, and α-SMA at different stages of skin wound healing. The JZX extract accelerates wound healing by reducing inflammation and inhibiting oxidative stress, regulating the PI3K/Akt signaling pathway, and promoting the expression of growth factors, suggesting that JZX has potential clinical applicability in wound treatment.
作为一种传统中药，交趾黄檀（JZX）自古以来就被用于治疗伤口。然而，JZX 加速伤口愈合的活性化合物和分子机制仍不清楚。在此，我们通过探讨 JZX 加速伤口愈合的主要活性化合物和关键分子机制来研究这一问题。采用 UPLC-Q-Orbitrap HRMS 分析方法对 JZX 的乙醇提取物进行分析，以鉴定其主要化合物。利用小鼠切除伤口模型评估 JZX 对伤口愈合的药理作用。采用网络药理学预测 JZX 加速伤口愈合的有效化合物及其相关信号转导通路。然后通过免疫组织化学分析验证预测的关键信号通路。通过分子对接分析确认活性化合物与治疗靶点之间的相互作用。JZX 可加速伤口愈合，改善组织质量，抑制炎症和氧化应激。此外，我们的研究结果表明，JZX 的活性成分，如丁布、杨梅素和芒柄花素，是促进伤口治疗的关键化合物。我们的研究还表明，JZX 通过调节 PI3K/Akt 信号通路并在皮肤伤口愈合的不同阶段诱导 TGF-β1、FGF2、VEGFA、ECM1 和 α-SMA 的表达来加速伤口愈合。JZX 提取物通过减少炎症和抑制氧化应激、调节 PI3K/Akt 信号通路以及促进生长因子的表达来加速伤口愈合，表明 JZX 在伤口治疗方面具有潜在的临床应用价值。
