Institute of Resource Biology and Biotechnology, Department of Biotechnology, College of Life Science and Technology, Huazhong University of Science and Technology, Wuhan 430074, China.
Key Laboratory of Molecular Biophysics of the Ministry of Education, College of Life Science and Technology and Center for Human Genome Research, Huazhong University of Science and Technology, Wuhan 430074, China.
J Ethnopharmacol. 2019 Jan 10;228:156-163. doi: 10.1016/j.jep.2018.08.008. Epub 2018 Aug 11.
In traditional Chinese medicine, the aerial parts of Aeschynomene indica L. (AIL) have been used for wound healing, and to treat urinary tract infection, hepatitis, enteritis, dysentery, nyctalopia, conjunctivitis, urticaria, and furuncle. However, no scientific investigation has been conducted on its wound healing potential.
To investigate the effects of AIL extract on wound healing, isolate the active constituent and reveal the possible mechanism of enhancing wound healing.
The circular excision wound healing model was used to evaluate in vivo wound-healing activity. Hematoxylin and eosin staining was applied to assess inflammatory cells infiltration, angiogenesis, fibroblast proliferation, collagen synthesis, collagen remodeling, and skin appendages generation. Sirius red-picric acid staining was employed for quantitative analysis of the ratio of collagen I/III. Immunohistochemical staining for CD68, CCR7 (CD197), CD163, TGF-β1 and α-SMA was performed to determine macrophages phenotypes transition (M1-to-M2) and prove the scar-improving effect of AIL on wound healing.
We successfully isolated the active constituent (Sub-Fr0.2) for wound healing from AIL extract, circular excision wound healing experiment and hematoxylin & eosin staining showed Sub-Fr0.2 has a significant promoting effect on wound healing. Results of sirius red-picric acid staining demonstrated a reduced ratio of collagen I/III in the Sub-Fr0.2 group as compared with the vehicle group. Immunohistochemical staining for CD68, CCR7 (CD197), and CD163 in the Sub-Fr0.2 group exhibited an elevated speed of macrophages transiting from M1 phenotype to M2 phenotype, when compared with the vehicle group. Besides, the expression of TGF-β1 and α-SMA were inhibited on wounds treated with the ointment containing Sub-Fr0.2.
Leaves of AIL and its active constituent (Sub-Fr0.2) effectively promoted wound healing and reduced scar formation, this efficacy might be exerted by accelerating macrophages phenotypes transition and inhibiting TGF-β1 and α-SMA expression.
在传统的中医理论中，田菁（AIL）的地上部分被用于治疗伤口愈合，以及治疗尿路感染、肝炎、肠炎、痢疾、夜盲症、结膜炎、荨麻疹和痈疽。然而，目前还没有对其伤口愈合潜力进行科学研究。
研究田菁提取物对伤口愈合的影响，分离活性成分并揭示增强伤口愈合的可能机制。
采用圆形切除伤口愈合模型评估体内伤口愈合活性。苏木精和伊红染色用于评估炎症细胞浸润、血管生成、成纤维细胞增殖、胶原合成、胶原重塑和皮肤附属物生成。天狼星红苦味酸染色用于定量分析胶原 I/III 的比值。免疫组织化学染色用于 CD68、CCR7（CD197）、CD163、TGF-β1 和 α-SMA，以确定巨噬细胞表型的转变（M1 到 M2），并证明 AIL 对伤口愈合的改善效果。
我们成功地从田菁提取物中分离出活性成分（Sub-Fr0.2）用于伤口愈合，圆形切除伤口愈合实验和苏木精和伊红染色显示 Sub-Fr0.2 对伤口愈合有显著的促进作用。天狼星红苦味酸染色结果表明，Sub-Fr0.2 组的胶原 I/III 比值较载体组降低。Sub-Fr0.2 组的 CD68、CCR7（CD197）和 CD163 的免疫组织化学染色显示，与载体组相比，巨噬细胞从 M1 表型向 M2 表型的转变速度加快。此外，用含有 Sub-Fr0.2 的软膏处理的伤口中 TGF-β1 和 α-SMA 的表达受到抑制。
田菁叶及其活性成分（Sub-Fr0.2）有效促进伤口愈合，减少瘢痕形成，这种功效可能是通过加速巨噬细胞表型转变和抑制 TGF-β1 和 α-SMA 表达来实现的。
