Yang Xiufeng, Sun Guolei, Xia Tian, Cha Muha, Zhang Lei, Pang Bo, Tang Qingming, Dou Huashan, Zhang Honghai
College of Life Science Qufu Normal University Qufu China.
Hulunbuir Academy of Inland Lakes in Northern Cold & Arid Areas Hulunbuir China.
Ecol Evol. 2022 Apr 19;12(4):e8866. doi: 10.1002/ece3.8866. eCollection 2022 Apr.
are widely distributed throughout the world and have undergone drastic physiological and phenotypic changes in response to their environment. However, little is known about the underlying genetic causes of these traits, especially . In this study, RNA-Seq was used to obtain a comprehensive dataset for multiple pooled tissues of corsac fox, and selection analysis of orthologous genes was performed to identify the genes that may be influenced by the low-temperature environment. More than 6.32 Gb clean reads were obtained and assembled into a total of 173,353 unigenes with an average length of 557 bp for corsac fox. Selective pressure analysis showed that 16 positively selected genes (PSGs) were identified in corsac fox, red fox, and arctic fox. Enrichment analysis of PSGs showed that the gene was enriched in several pathways related to the low-temperature response and might play a key role in response to environmental stimuli of foxes. In addition, several positively selected genes were related to DNA damage repair ( and ), innate immunity ( and ), and the respiratory chain (), and these positively selected genes might play a role in adaptation to harsh wild fox environments. The results of common orthologous gene analysis showed that gene flow or convergent evolution might be an important factor in promoting regional differentiation of foxes. Our study provides a valuable transcriptomic resource for the evolutionary history of the corsac fox and the adaptations to the extreme environments.
它们广泛分布于世界各地，并因环境变化而经历了剧烈的生理和表型变化。然而，对于这些特征的潜在遗传原因知之甚少，尤其是……在本研究中，利用RNA测序技术获得了藏狐多个混合组织的综合数据集，并对直系同源基因进行了选择分析，以确定可能受低温环境影响的基因。藏狐共获得了超过6.32Gb的 clean reads，并组装成了总共173,353个单基因，平均长度为557bp。选择压力分析表明，在藏狐、赤狐和北极狐中鉴定出了16个正选择基因（PSGs）。对PSGs的富集分析表明，该基因在几个与低温反应相关的途径中富集，可能在狐狸对环境刺激的反应中起关键作用。此外，一些正选择基因与DNA损伤修复（……和……）、先天免疫（……和……）以及呼吸链（……）有关，这些正选择基因可能在适应恶劣的野生狐狸环境中发挥作用。共同直系同源基因分析结果表明，基因流或趋同进化可能是促进狐狸区域分化的重要因素。我们的研究为藏狐的进化历史及其对极端环境的适应性提供了宝贵的转录组资源。
