基于超高效液相色谱-四极杆飞行时间质谱联用技术对中药方剂利胆汤中化合物的化学表征及代谢轮廓分析

Chemical Characterization and Metabolic Profiling of the Compounds in the Chinese Herbal Formula Li Chang Decoction by UPLC-QTOF/MS.

作者信息

Lin Baofu, Guo Shaoju, Hong Xinxin, Jiang Xiaoyan, Li Haiwen, Li Jingwei, Guo Linglong, Li Mianli, Chen Jianping, Huang Bin, Xu Yifei

机构信息

Shenzhen Traditional Chinese Medicine Hospital, The Fourth Clinical Medical College of Guangzhou University of Chinese Medicine, Shenzhen 518033, China.

出版信息

Evid Based Complement Alternat Med. 2022 Apr 13;2022:1322751. doi: 10.1155/2022/1322751. eCollection 2022.

BACKGROUND

Li Chang decoction (LCD), a Chinese medicine formula, is commonly used to treat ulcerative colitis (UC) in clinics.

PURPOSE

This study aimed to identify the major components in LCD and its prototype and metabolic components in rat biological samples.

METHODS

The chemical constituents in LCD were identified by establishing a reliable ultra-performance liquid chromatography coupled with quadrupole time-of-flight tandem mass spectrometry (UPLC-QTOF/MS) method. Afterwards, the rats were orally administered with LCD, and the biological samples (plasma, urine, and feces) were collected for further analyzing the effective compounds in the treatment of UC.

RESULT

A total of 104 compounds were discriminated in LCD, including 26 flavonoids, 20 organic acids, 20 saponins, 8 amino acids, 5 oligosaccharides, 5 tannins, 3 lignans, 2 alkaloids, and 15 others (nucleosides, glycosides, esters, etc.). About 50 prototype and 94 metabolic components of LCD were identified in biological samples. In total, 29 prototype components and 22 metabolic types were detected in plasma. About 27 prototypes and 96 metabolites were discriminated in urine, and 34 prototypes and 18 metabolites were identified in feces.

CONCLUSION

The flavonoids, organic acids, and saponins were the major compounds of LCD, and this study promotes the further pharmacokinetic and pharmacological evaluation of LCD.

背景

中药方剂李昌汤（LCD）在临床上常用于治疗溃疡性结肠炎（UC）。

目的

本研究旨在鉴定LCD中的主要成分及其原型，以及大鼠生物样品中的代谢成分。

方法

通过建立可靠的超高效液相色谱-四极杆飞行时间串联质谱（UPLC-QTOF/MS）方法来鉴定LCD中的化学成分。之后，给大鼠口服LCD，并收集生物样品（血浆、尿液和粪便）以进一步分析治疗UC中的有效化合物。

结果

在LCD中总共鉴别出104种化合物，包括26种黄酮类化合物、20种有机酸、20种皂苷、8种氨基酸、5种低聚糖、5种单宁、3种木脂素、2种生物碱和15种其他化合物（核苷、糖苷、酯等）。在生物样品中鉴定出约50种LCD的原型成分和94种代谢成分。总共在血浆中检测到29种原型成分和22种代谢类型。在尿液中鉴别出约27种原型成分和96种代谢产物，在粪便中鉴定出34种原型成分和18种代谢产物。