Singh R D, Patel K A, Patel J B, Pandya S J, Patel P S
Department of Biochemistry, All India Institute of Medical Sciences, Khandheri, Rajkot, Gujarat 360110 India.
Molecular Oncology Laboratory, Cancer Biology Department, The Gujarat Cancer and Research Institute, Asarwa, Ahmedabad, Gujarat 380 016 India.
Indian J Clin Biochem. 2022 Apr;37(2):178-184. doi: 10.1007/s12291-021-00958-1. Epub 2021 Feb 12.
Association of smokeless tobacco (SLT) with oral cancer is well documented. The nitrosamines of SLT are metabolically bioactivated by . Therefore, in this pilot study, we investigated association of CYP1A1 expression with polymorphism, clinicopathological variables and outcome in exclusive SLT chewers. Semiquantitative RT-PCR analysis on RNA isolated from peripheral blood lymphocytes of 65 histopathologically confirmed cases revealed increased CYP1A1 mRNA expression in Msp I () and Ile/Val () polymorphic variants. A higher trend of CYP1A1 mRNA levels in invasive and recurrent tumors which also associated with shorter disease free and overall survival was seen. Odds ratio analysis indicated the association of higher CYP1A1 mRNA levels with disease aggressiveness i.e. metastasis and invasion. Study revealed wider oncogenic role of in oral cancer patients and justifies further studies and analysis in a large cohort.
无烟烟草（SLT）与口腔癌的关联已有充分记录。SLT中的亚硝胺通过……进行代谢生物活化。因此，在本初步研究中，我们调查了CYP1A1表达与多态性、临床病理变量以及单纯SLT咀嚼者预后之间的关联。对65例经组织病理学确诊病例的外周血淋巴细胞分离的RNA进行半定量RT-PCR分析，结果显示在Msp I（）和Ile/Val（）多态性变体中CYP1A1 mRNA表达增加。在侵袭性和复发性肿瘤中观察到CYP1A1 mRNA水平有更高的趋势，这也与较短的无病生存期和总生存期相关。优势比分析表明较高的CYP1A1 mRNA水平与疾病侵袭性即转移和侵袭有关。研究揭示了……在口腔癌患者中更广泛的致癌作用，并证明在大型队列中进行进一步研究和分析是合理的。
