Amigorena S, Moncuit J, Fridman W H, Teillaud J L
J Immunol Methods. 1987 Feb 26;97(1):57-64. doi: 10.1016/0022-1759(87)90105-0.
A microtiter plate assay to detect the effect of immunoglobulin binding factor (IBF) on Ig secretion by hybridoma B cells is described. This technique permits the analysis of Ig secretion by only 200-400 hybridoma B cells using a PFC assay and an ELISA, and therefore increases the IBF/cell number ratio. This increase allows the detection of a strong IBF-mediated inhibitory effect on Ig secretion by hybridoma B cells, which is otherwise difficult to obtain reproducibly. The technique is simple, does not require any transferring step and is convenient, since it permits large numbers of samples to be tested. It can be extended to test IBF for all isotypes or other lymphokines that affect Ig secretion.
本文描述了一种用于检测免疫球蛋白结合因子（IBF）对杂交瘤B细胞分泌免疫球蛋白（Ig）影响的微量滴定板检测方法。该技术仅使用200 - 400个杂交瘤B细胞，通过空斑形成细胞（PFC）检测和酶联免疫吸附测定（ELISA）来分析Ig分泌，从而提高了IBF与细胞数量的比例。这种增加使得能够检测到IBF对杂交瘤B细胞Ig分泌的强烈抑制作用，否则很难重复获得这种效果。该技术简单，不需要任何转移步骤，且方便实用，因为它允许检测大量样本。它可以扩展用于检测所有免疫球蛋白亚型的IBF或其他影响Ig分泌的淋巴因子。
