Teillaud J L, Mathiot C, Amigorena S, Brunati S, Moncuit J, Fridman W H
Laboratoire d'Immunologie Cellulaire et Clinique, Unité INSERM 255, Institut Curie, Paris, France.
Cancer Detect Prev. 1988;12(1-6):115-23.
Immunoglobulin-binding factors (IBF), which are cytokines able to suppress the immunoglobulin production by normal and transformed B cells, have been tested for their ability to interfere with hybridoma B-cell growth. Coculture experiments performed in soft agar between hybridoma B cells and hybridoma T cells secreting IgG-BF indicated that IgG-BF act as a growth regulatory molecule able to inhibit strongly the development of hybridoma B-cell colonies; this cytostatic effect, which appeared not to be a direct cytotoxic effect, was confirmed when using semi-purified IgG-BF in both soft agar and liquid medium cultures of hybridoma B cells. Thus, IBF appear to act as both immunoregulatory and growth regulatory factors. Some of the molecular consequences of this dual effect and its possible role in certain B-cell lymphoproliferative diseases such as multiple myeloma are discussed.
免疫球蛋白结合因子（IBF）是一类能够抑制正常和转化B细胞产生免疫球蛋白的细胞因子，已对其干扰杂交瘤B细胞生长的能力进行了测试。在软琼脂中进行的杂交瘤B细胞与分泌IgG - BF的杂交瘤T细胞之间的共培养实验表明，IgG - BF作为一种生长调节分子，能够强烈抑制杂交瘤B细胞集落的形成；当在杂交瘤B细胞的软琼脂和液体培养基培养中使用半纯化的IgG - BF时，证实了这种细胞抑制作用似乎不是直接的细胞毒性作用。因此，IBF似乎同时作为免疫调节因子和生长调节因子发挥作用。本文讨论了这种双重作用的一些分子后果及其在某些B细胞淋巴增殖性疾病如多发性骨髓瘤中的可能作用。
