Teillaud J L, Amigorena S, Moncuit J, Sautès C, Fridman W H
Laboratorie d'Immunologie Cellulaire et Clinique, INSERM U.255, Institut Curie, Paris, France.
Immunol Lett. 1987 Nov;16(2):139-44. doi: 10.1016/0165-2478(87)90121-0.
Mouse immunoglobulin G-binding factors (IgG-BF) produced either by activated T cells (ATC) or by hybridoma T cells (T2D4) directly inhibit the in vitro IgG secretion by hybridoma B cells. This inhibition affects IgG1, IgG2a and IgG2b and can be detected as early as after 2 h incubation of the cells with IgG-BF eluted from non-equilibrium pH gradient electrophoresis gels. Moreover, IgG-BF also exert a strong growth-inhibitory effect on hybridoma B cells without any detectable immediate cytotoxicity. These results provide an experimental basis to analyze the molecular and biological effects induced by IgG-BF on B cells.
由活化T细胞（ATC）或杂交瘤T细胞（T2D4）产生的小鼠免疫球蛋白G结合因子（IgG-BF）可直接抑制杂交瘤B细胞的体外IgG分泌。这种抑制作用影响IgG1、IgG2a和IgG2b，并且早在细胞与从非平衡pH梯度电泳凝胶洗脱的IgG-BF孵育2小时后即可检测到。此外，IgG-BF对杂交瘤B细胞也具有很强的生长抑制作用，且没有任何可检测到的直接细胞毒性。这些结果为分析IgG-BF对B细胞诱导的分子和生物学效应提供了实验依据。
