Kreuzfelder E, Shen G, Rodeck U, König E, Luboldt W, Keinecke H O, Brockmeyer N, Scheiermann N
J Immunol Methods. 1987 Mar 12;97(2):251-8. doi: 10.1016/0022-1759(87)90467-4.
Lymphocyte subpopulations were determined in blood samples from blood donors (40 women and 45 men) using immunofluorescence microscopy and flow cytometric methodologies. The study demonstrates the value of both methods for the enumeration of lymphocyte subpopulations. The advantages of employing an automated flow cytometer system are better precision and speed. The automated systems require a large initial technical and financial burden and are therefore probably destined to be reserved for the larger laboratory. There is a need for an adequate lymphocyte standard which shows little variation between aliquots and can be used for interlaboratory comparisons.
使用免疫荧光显微镜和流式细胞术方法，对献血者（40名女性和45名男性）的血样中的淋巴细胞亚群进行了测定。该研究证明了这两种方法在淋巴细胞亚群计数方面的价值。采用自动流式细胞仪系统的优点是具有更高的精度和速度。自动系统需要巨大的初始技术和资金负担，因此可能注定只能供较大的实验室使用。需要有一个合适的淋巴细胞标准品，其不同分装之间差异很小，可用于实验室间的比较。
