Li P L
Department of Immunology, Tongji Medical University, Wuhan.
J Tongji Med Univ. 1991;11(2):88-92. doi: 10.1007/BF02888094.
The changes of the intracellular inositol-1,4,5-triphosphate (IP3) associated with the expression of the interleukin 2 receptor (IL-2R) were studied. In resting lymphocytes, IL-2 did not alter intracellular concentration of IP3, but Con A caused an increase in IP3 by 45%. In IL-2 sensitive T cells, which expressed IL-2R by 83%, the change of intracellular IP3 was dependent upon IL-2 concentration. The IP3 increased at IL-2 concentrations of 10 and 50 U/ml, and the maximal response of 60% was found at the concentration of 50 U/ml. At IL-2 concentration of 100 U/ml no increase in IP3 was observed. After binding of anti-Tac McAb to IL-2R of T lymphocytes the increase in IP3 at IL-2 concentrations of 10 and 50 U/ml was significantly attenuated. It has been suggested that IL-2 could induce the changes of intracellular IP3 of the human peripheral blood T cells, which is related to the IL-2 concentrations incubated with T cells and the expression of IL-2R on T lymphocyte.
研究了与白细胞介素2受体（IL-2R）表达相关的细胞内肌醇-1,4,5-三磷酸（IP3）的变化。在静息淋巴细胞中，IL-2不会改变细胞内IP3的浓度，但刀豆蛋白A会使IP3增加45%。在83%表达IL-2R的IL-2敏感T细胞中，细胞内IP3的变化取决于IL-2的浓度。在IL-2浓度为10和50 U/ml时IP3增加，在50 U/ml浓度时发现最大反应为60%。在IL-2浓度为100 U/ml时未观察到IP3增加。抗Tac单克隆抗体与T淋巴细胞的IL-2R结合后，在IL-2浓度为10和50 U/ml时IP3的增加显著减弱。有人提出，IL-2可诱导人外周血T细胞细胞内IP3的变化，这与与T细胞孵育的IL-2浓度以及T淋巴细胞上IL-2R的表达有关。
