von dem Borne A E, Verheugt F W, Oosterhof F, von Riesz E, de la Rivière A B, Engelfriet C P
Br J Haematol. 1978 Jun;39(2):195-207. doi: 10.1111/j.1365-2141.1978.tb01089.x.
Immunofluorescence tests on platelets have always been hampered by nonspecific fluorescence caused by non-immunological binding of plasma proteins to the platelet membrane. It was found that this could be easily overcome by fixation of the cells with paraformaldehyde (PFA). By using PFA-fixed platelets, a simple method for the detection of platelet antibodies, the platelet suspension immunofluorescence test (PSIFT) was developed. PFA fixation did not alter or inactivate the platelet antigens tested. Platelet-reactive antibodies detected specifically with the PSIFT included platelet-specific agglutinins of the IgM class, non-agglutinating platelet-specific antibodies of the IgG class, drug-dependent platelet antibodies, HLA antibodies, as well as anti-A and anti-B antibodies. The sensitivity of the new test was satisfactory, as was its reproducibility. Measurement of platelet immunofluorescence was possible in a continuous flow microfluorometer, making a principle, quantitation of platelet antibodies and antigens possible.
血小板的免疫荧光检测一直受到血浆蛋白与血小板膜非免疫性结合所导致的非特异性荧光的干扰。研究发现，用多聚甲醛（PFA）固定细胞可轻松克服这一问题。通过使用PFA固定的血小板，开发出了一种检测血小板抗体的简单方法，即血小板悬液免疫荧光试验（PSIFT）。PFA固定并未改变或灭活所检测的血小板抗原。用PSIFT特异性检测到的血小板反应性抗体包括IgM类血小板特异性凝集素、IgG类非凝集性血小板特异性抗体、药物依赖性血小板抗体、HLA抗体以及抗A和抗B抗体。新试验的灵敏度令人满意，其重复性也很好。在连续流动式微量荧光计中可以测量血小板免疫荧光，从原理上讲，这使得血小板抗体和抗原的定量成为可能。
