Boisvert A, MacPherson B R
Am J Clin Pathol. 1983 Dec;80(6):839-43. doi: 10.1093/ajcp/80.6.839.
The development of sensitive immunofluorescent technics for the detection of platelet antibodies has stimulated renewed interest in the field of platelet immunology. However, the analysis of multispecific sera containing both HLA and platelet-specific antibodies requires cumbersome absorption technics and relatively large quantities of serum. In this report we describe the feasibility of screening sera from multiparous women and multitransfused patients for platelet-specific antibodies using blocking inhibition with a murine monoclonal antibody against HLA A, B, and C antigens followed by platelet immunofluorescence. Platelet immunofluorescence detected anti-A antibodies in sera from some blood group O donors. Known HLA antibodies usually were detectable by platelet immunofluorescence, but false-positive reactions occurred frequently. Analysis of sera from recipients of multiple platelet transfusions revealed that 47/78 (60.2%) were positive by platelet immunofluorescence. After blocking inhibition-persistent fluorescence was observed with 11/29 sera (37.9%), suggesting the presence of platelet-specific antibodies. Absorption studies performed on two of these sera revealed that antibody activity was removed by absorption with platelets but not lymphocytes. Platelet immunofluorescence combined with monoclonal antibody blocking inhibition proved to be a valuable tool to distinguish between HLA and platelet-specific antibodies.
用于检测血小板抗体的灵敏免疫荧光技术的发展激发了人们对血小板免疫学领域的新兴趣。然而，对同时含有HLA和血小板特异性抗体的多特异性血清进行分析需要繁琐的吸收技术以及相对大量的血清。在本报告中，我们描述了通过使用抗HLA A、B和C抗原的鼠单克隆抗体进行阻断抑制，随后进行血小板免疫荧光，来筛查多产妇女和多次输血患者血清中血小板特异性抗体的可行性。血小板免疫荧光在一些O型血供者的血清中检测到了抗A抗体。已知的HLA抗体通常可通过血小板免疫荧光检测到，但假阳性反应频繁发生。对多次接受血小板输血的受者血清进行分析发现，78份血清中有47份（60.2%）通过血小板免疫荧光呈阳性。在进行阻断抑制后，29份血清中有11份（37.9%）观察到持续荧光，提示存在血小板特异性抗体。对其中两份血清进行的吸收研究表明，抗体活性可通过血小板吸收而去除，但不能被淋巴细胞吸收。血小板免疫荧光结合单克隆抗体阻断抑制被证明是区分HLA和血小板特异性抗体的有价值工具。
