Andersen E, Bashir H, Archer G T
Vox Sang. 1981 Jan;40(1):44-47. doi: 10.1111/j.1423-0410.1981.tb00667.x.
This study reports two modifications of the Platelet Suspension Immunofluorescence Test which make it more convenient to use for the rapid identification of platelet-specific antibodies and for screening individuals if platelet donors of a particular type are urgently required. Platelets frozen both before and after paraformaldehyde (PFA) fixation were compared with fresh PFA-fixed platelets using anti-PLA1 and anti-HLA antisera. Further, a comparison was made between the results of the test when performed in tissue culture trays or in tubes as originally described. Platelets, prefixed with PFA and then frozen appeared similar in all respects to fresh PFA-fixed platelets with no loss of antigenicity and no non-specific fluorescence. Although platelets fixed after frozen storage were also satisfactory, they were less convenient to use and lost some brilliance in staining. When the test was performed in tissue culture trays, there was no loss in sensitivity, and the volume of antiserum used was halved. However, the main advantage was the efficiency and ease with which the test could be performed, especially when handling large numbers of samples.
本研究报告了血小板悬浮免疫荧光试验的两种改进方法，这使其在快速鉴定血小板特异性抗体以及在急需特定类型血小板供体时筛选个体方面使用起来更加方便。使用抗PLA1和抗HLA抗血清，将多聚甲醛（PFA）固定前后冷冻的血小板与新鲜PFA固定的血小板进行了比较。此外，还对按照最初描述在组织培养板或试管中进行试验的结果进行了比较。先用PFA固定然后冷冻的血小板在各方面都与新鲜PFA固定的血小板相似，抗原性无损失且无非特异性荧光。虽然冷冻保存后固定的血小板也令人满意，但使用起来不太方便，染色时会损失一些亮度。当在组织培养板中进行试验时，灵敏度没有损失，抗血清的用量减半。然而，主要优点是试验执行的效率和简便性，尤其是在处理大量样本时。
