Thiam Laty Gaye, Mangou Khadidiatou, Ba Aboubacar, Mbengue Alassane, Bei Amy K
G4 - Malaria Experimental Genetic Approaches & Vaccines, Pôle Immunophysiopathologie et Maladies Infectieuses, Institut Pasteur de Dakar, Dakar, Senegal.
G4 - Malaria Experimental Genetic Approaches & Vaccines, Pôle Immunophysiopathologie et Maladies Infectieuses, Institut Pasteur de Dakar, Dakar, Senegal; Department of Epidemiology of Microbial Diseases, Yale School of Public Health, New Haven, CT 06510, USA.
Trends Parasitol. 2022 Jul;38(7):558-571. doi: 10.1016/j.pt.2022.03.005. Epub 2022 Apr 22.
The ambitious goal of malaria elimination requires an in-depth understanding of the parasite's biology to counter the growing threat of antimalarial resistance and immune evasion. Timely assessment of the functional impact of antigenic diversity in the early stages of vaccine development will be critical for achieving the goal of malaria control, elimination, and ultimately eradication. Recent advances in targeted genome editing enabled the functional validation of resistance-associated markers in Plasmodium falciparum, the deadliest malaria-causing pathogen and strain-specific immune neutralization. This review explores recent advances made in leveraging genome editing to aid the functional evaluation of Plasmodium diversity and highlights how these techniques can assist in prioritizing both therapeutic and vaccine candidates.
消除疟疾这一宏伟目标需要深入了解疟原虫的生物学特性，以应对日益增长的抗疟药物耐药性和免疫逃避威胁。在疫苗研发早期及时评估抗原多样性的功能影响，对于实现疟疾控制、消除乃至最终根除的目标至关重要。靶向基因组编辑技术的最新进展使得对恶性疟原虫（最致命的致病疟原虫）中与耐药性相关的标记物以及菌株特异性免疫中和进行功能验证成为可能。本综述探讨了利用基因组编辑在辅助疟原虫多样性功能评估方面取得的最新进展，并强调了这些技术如何有助于确定治疗和疫苗候选物的优先级。
