Quansah Elvis, Chen Yihuan, Yang Shijie, Wang Junyan, Sun Danhong, Zhao Yangxi, Chen Ming, Yu Li, Zhang Chao
Anhui Provincial Laboratory of Microbiology and Parasitology, Anhui Key Laboratory of Zoonoses, Department of Microbiology and Parasitology, School of Basic Medical Sciences, Anhui Medical University, Hefei, China.
The Second Clinical Medical College, Anhui Medical University, Hefei, China.
Front Microbiol. 2023 Jan 25;14:1076947. doi: 10.3389/fmicb.2023.1076947. eCollection 2023.
Malaria caused by is still a serious public health problem. Genomic editing is essential to understand parasite biology, elucidate mechanical pathways, uncover gene functions, identify novel therapeutic targets, and develop clinical diagnostic tools. Recent advances have seen the development of genomic diagnostic technologies and the emergence of genetic manipulation toolbox comprising a host of several systems for editing the genome of at the DNA, RNA, and protein level. Genomic manipulation at the RNA level is critical as it allows for the functional characterization of several transcripts. Of notice, some developed artificial RNA genome editing tools hinge on the endogenous RNA interference system of . However, lacks a robust RNAi machinery, hampering the progress of these editing tools. CRISPR-Cas13, which belongs to the VI type of the CRISPR system, can specifically bind and cut RNA under the guidance of crRNA, with no or minimal permanent genetic scar on genes. This review summarizes CRISPR-Cas13 system from its discovery, classification, principle of action, and diagnostic platforms. Further, it discusses the application prospects of Cas13-based systems in and highlights its advantages and drawbacks.
由[具体病原体]引起的疟疾仍然是一个严重的公共卫生问题。基因组编辑对于理解寄生虫生物学、阐明作用机制、揭示基因功能、识别新的治疗靶点以及开发临床诊断工具至关重要。最近的进展包括基因组诊断技术的发展以及一系列用于在DNA、RNA和蛋白质水平编辑[具体病原体]基因组的遗传操作工具箱的出现。RNA水平的基因组操作至关重要，因为它能够对多个转录本进行功能表征。值得注意的是，一些已开发的人工RNA基因组编辑工具依赖于[具体病原体]的内源性RNA干扰系统。然而，[具体病原体]缺乏强大的RNA干扰机制，阻碍了这些编辑工具的进展。CRISPR-Cas13属于CRISPR系统的VI型，能够在crRNA的引导下特异性结合并切割RNA，对基因没有或只有最小程度的永久性遗传损伤。本综述从CRISPR-Cas13系统的发现、分类、作用原理和诊断平台等方面进行了总结。此外，还讨论了基于Cas13的系统在[具体病原体]中的应用前景，并突出了其优缺点。
