Döller P C, Döller G, Gerth H J
Med Microbiol Immunol. 1987;176(1):27-35. doi: 10.1007/BF00189406.
We report on results obtained with a direct immunofluorescence test for subtype-specific identification of influenza virus in detached cells of MDCK cultures after inoculation of 281 clinical specimens from patients with influenza-like disease. Influenza virus antibodies were produced in eggs from immunized hens and labelled with FITC. In 157 cases CPE was found in MDCK cells. A total of 57 cases of influenza A (H3N2), 86 cases of influenza A (H1N1), and 14 cases of influenza B were identified. In 33 cases of influenza A (H1N1) infection with massive CPE guinea pig but not chicken erythrocytes were agglutinated by the cell culture supernatants. The single step immunofluorescence test described proved easy to perform and results were obtained within 1 h after CPE was observed in contrast to the conventional HIT which is very time-consuming.
我们报告了在接种281份来自流感样疾病患者的临床标本后，对MDCK培养物的分离细胞进行流感病毒亚型特异性鉴定的直接免疫荧光试验所获得的结果。流感病毒抗体由免疫母鸡的鸡蛋产生并用异硫氰酸荧光素标记。在157例中，MDCK细胞出现细胞病变效应（CPE）。共鉴定出57例甲型流感（H3N2）、86例甲型流感（H1N1）和14例乙型流感。在33例出现大量CPE的甲型流感（H1N1）感染病例中，细胞培养上清液能凝集豚鼠红细胞而非鸡红细胞。所描述的单步免疫荧光试验操作简便，与非常耗时的传统血凝抑制试验（HIT）相比，在观察到CPE后1小时内即可获得结果。
