LE Yilin, He Xing, Sun Jianzhong
Biofuels Institute, School of the Environment and Safety Engineering, Jiangsu University, Zhenjiang 212013, Jiangsu, China.
Sheng Wu Gong Cheng Xue Bao. 2022 Apr 25;38(4):1475-1489. doi: 10.13345/j.cjb.210827.
The diverse thermophilic strains of , serving as unique platforms with a broad range of application in biofuels and chemicals, have received wide attention from scholars and practitioners. Although biochemical experiments and genome sequences have been reported for a variety of strains, an efficient genetic manipulation system remains to be established for revealing the biosynthetic pathways of . In line with this demand, the clustered regularly interspaced short palindromic repeat (CRISPR)/CRISPR-associated (Cas) systems for editing, regulating and targeting genomes have been well developed in thermophiles. Here, we reviewed and discussed the current status, associated challenges, and future perspectives of the construction of thermostable CRISPR/Cas9 genome editing systems for some representative species. The establishment, optimization, and application of thermostable CRISPR/Cas genome editing systems would potentially provide a foundation for further genetic modification of thermophilic bacteria.
作为在生物燃料和化学品领域具有广泛应用的独特平台，嗜热菌的多种嗜热菌株受到了学者和从业者的广泛关注。尽管已经报道了多种嗜热菌菌株的生化实验和基因组序列，但仍有待建立一个高效的遗传操作体系来揭示嗜热菌的生物合成途径。为满足这一需求，用于编辑、调控和靶向基因组的成簇规律间隔短回文重复序列（CRISPR）/CRISPR相关蛋白（Cas）系统在嗜热菌中得到了很好的发展。在此，我们综述并讨论了一些代表性嗜热菌物种构建热稳定CRISPR/Cas9基因组编辑系统的现状、相关挑战及未来展望。热稳定CRISPR/Cas基因组编辑系统的建立、优化及应用可能为嗜热细菌的进一步基因改造提供基础。
