利用红外光谱法对铜绿假单胞菌和鲍曼不动杆菌周围的气体进行分类
Classification of gases around Pseudomonas aeruginosa and Acinetobacter baumannii by infrared spectroscopy.
作者信息
Yamamoto Masato, Arata Satoru, Fukuchi Kunihiko, Honda Hidehiko, Kobayashi Hirokazu, Inagaki Masahiro
机构信息
Department of Chemistry, College of Arts and Sciences, Showa University, 4562 Kamiyoshida, Fujiyoshida-city, Yamanashi 403-0005, Japan.
Department of Chemistry, College of Arts and Sciences, Showa University, 4562 Kamiyoshida, Fujiyoshida-city, Yamanashi 403-0005, Japan; Center for Biotechnology, Showa University, 1-5-8 Hatanodai, Shinagawa-ku, Tokyo 142-8555, Japan.
出版信息
J Microbiol Methods. 2022 May;196:106474. doi: 10.1016/j.mimet.2022.106474. Epub 2022 Apr 25.
Gas samples were collected from the air surrounding single and mixed laboratory cultures, and preliminary data on human breath samples were also obtained. The infrared spectra of a variety of gasses were measured at high resolution (0.5 cm) to obtain information about the infrared absorption bands to be used as indicators. These key bands enable bacterial classification, and the discrimination rates can be improved by observing multiple infrared absorptions. The air around Pseudomonas aeruginosa was distinguished from the other gas samples by the infrared absorptions at wavenumbers of 778.4 cm and 2213.2 cm. For Acinetobacter baumannii, infrared absorptions at 1215.0 cm and 2982.3 cm were used; furthermore, adding those at 4768.7 cm and 5353.8 cm was shown to improve identification.
从单一和混合实验室培养物周围的空气中采集气体样本，并获取了有关人类呼吸样本的初步数据。以高分辨率（0.5厘米）测量了多种气体的红外光谱，以获取有关用作指标的红外吸收带的信息。这些关键谱带能够实现细菌分类，并且通过观察多个红外吸收可以提高鉴别率。铜绿假单胞菌周围的空气通过波数为778.4厘米和2213.2厘米处的红外吸收与其他气体样本区分开来。对于鲍曼不动杆菌，使用了1215.0厘米和2982.3厘米处的红外吸收；此外，添加4768.7厘米和5353.8厘米处的吸收可提高识别率。
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