Department of Biotechnology, K. S. Rangasamy College of Arts and Science (Autonomous), Tiruchengode, 637 215, Namakkal, Tamil Nadu, India.
Department of Biotechnology, K. S. Rangasamy College of Arts and Science (Autonomous), Tiruchengode, 637 215, Namakkal, Tamil Nadu, India; Department of Science, School of Health & Life Science, Teesside University, Middlesbrough, Tees Valley, TS13BX, England, UK.
Environ Res. 2022 Sep;212(Pt C):113323. doi: 10.1016/j.envres.2022.113323. Epub 2022 Apr 23.
The present study focused on synthesizing ZnO nanoparticles (NPs) and CuO NPs using Elaeagnus indica leaf extract as reducing and stabilizing agents using Zn(OCCH) and CuSO, respectively, for the first time. We have confirmed the formation of aggregated ZnO NPs and CuO NPs with phytochemicals by various spectral analyses and electron microscopy studies. The size of synthesized ZnO NPs and CuO NPs were in the range of 20-30 nm and 30-40 nm, respectively. The antimicrobial activity of ZnO NPs at 75 μg concentration is superior against Salmonella typhimurium, Klebsiella pneumonia, Bacillus subtilis, Staphylococcus epidermidis, and Aspergillus niger. While CuO nanoparticles with 75 μg concentration effectively inhibited S. typhimurium, B. subtilis, S. epidermidis, and A. niger. Phytochemicals and reactive oxygen species generated by the prepared NPs may account for the antimicrobial effects observed. The photodegradation of methylene blue by ZnO NPs and CuO NPs was 91% and 76%, respectively, for 6 h of sunlight exposure. CuO NPs and ZnO NPs have different intrinsic properties and phytochemical compositions; hence ZnO NPs photodegrade faster than CuO NPs even though ZnO has higher bandgap energy than CuO. Consequently, CuO and ZnO NPs produced from E. indica leaf extract might be utilized as antimicrobials and photocatalysts in the future.
本研究首次使用沙枣叶提取物作为还原和稳定剂，分别使用 Zn(OCCH) 和 CuSO 合成 ZnO 纳米粒子 (NPs) 和 CuO NPs。通过各种光谱分析和电子显微镜研究，我们已经证实了具有植物化学物质的聚集 ZnO NPs 和 CuO NPs 的形成。合成的 ZnO NPs 和 CuO NPs 的尺寸分别在 20-30nm 和 30-40nm 范围内。75μg 浓度的 ZnO NPs 对肠炎沙门氏菌、肺炎克雷伯菌、枯草芽孢杆菌、表皮葡萄球菌和黑曲霉具有优异的抗菌活性。而 75μg 浓度的 CuO 纳米粒子能有效抑制肠炎沙门氏菌、枯草芽孢杆菌、表皮葡萄球菌和黑曲霉。制备的 NPs 产生的植物化学物质和活性氧可能是观察到的抗菌作用的原因。在 6 小时的阳光照射下，ZnO NPs 和 CuO NPs 对亚甲基蓝的光降解率分别为 91%和 76%。CuO NPs 和 ZnO NPs 具有不同的固有性质和植物化学成分；因此，即使 ZnO 的能带隙能量高于 CuO，ZnO NPs 的光降解速度也比 CuO NPs 快。因此，未来可能会将从沙枣叶中提取的 CuO 和 ZnO NPs 用作抗菌剂和光催化剂。
